|Table of Contents|

Preliminary Analysis of Subcellular Localization and Pathogenicity of Little cherry virus 1 Protein P3.5

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2023年16
Page:
48-52
Research Field:
Publishing date:

Info

Title:
Preliminary Analysis of Subcellular Localization and Pathogenicity of Little cherry virus 1 Protein P3.5
Author(s):
LI Yuanyuan1; YU Qiuyue1; SHANG Qinwang1; WANG Hongtao2; LI Qingliang1; WANG Deya1
(1.College of Life Sciences，Zaozhuang University，Zaozhuang,Shandong 277160；2.Yicheng Agricultural High-tech Industry Demonstration Zone，Zaozhuang，Shandong 277160)
Keywords:
Little cherry virus 1; P3.5 protein; subcellular localization; pathogenicity
PACS:
-
DOI:
10.11937/bfyy.20224507
Abstract:
Little cherry virus 1 (LChV-1) was as research subject in this study.The subcellular localization and pathogenicity of P3.5 protein were studied by using molecular biology methods,in order to provide reference for the studying of the very small proteins encoded by plant viruses.The results showed that,1) P3.5 was encoded by ORF2 of LChV-1,a small hydrophobic protein of unknown function.Primarily,P3.5 was cloned from an isolate of LChV-1 from Tai′an,Shandong province using specific primers by RT-PCR,which was 96 bp in length and encoded a protein of approximately 3.5 kD with a transmembrane domain.2) Subcellular localization analysis by tobacco leaves showed that P3.5 localized in cytomembrane and nuclear membrane.3) To further clarify the pathogenicity of P3.5,a potato virus X (PVX)-based vector was introduced and PVX-P3.5 was constructed.The PVX-P3.5 was transformed into Agrobacterium tumefaciens GV3101 and infiltrated into the N.benthamiana.Slight mosaic symptom was observed in the plants inoculated by PVX 14 days later.Meanwhile,when the plants inoculated by PVX-P3.5,the plants didn′t have visible symptoms.It demonstrated that heterologous expression of P3.5 in N.benthamiana didn′t enhanced the pathogenicity of PVX,and not be related to the symptom-formation.

References:

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Last Update: 2023-09-27