|Table of Contents|

Study on Genetic Relationship and DNA Fingerprint of Ornamental Pomegranate Cultivars Based on ISSR Markers

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2023年15
Page:
59-65
Research Field:
Publishing date:

Info

Title:
Study on Genetic Relationship and DNA Fingerprint of Ornamental Pomegranate Cultivars Based on ISSR Markers
Author(s):
ZHU Wei1; WU Zhiping2; LI Xue3; WANG Qingjun1; YUAN Lixin2; MA Li2
（1.Zaozhuang Agricultural and Mechanical Technology Promotion Center,Zaozhuang, Shangdong 277800;2.Life Science Department of Zaozhuang College,Zaozhuang,Shangdong 277160;3.Zaozhuang Agricultural and Rural Development Center,Zaozhuang,Shandong 277800）
Keywords:
ornamental pomegranate; ISSR; genetic relationship; DNA fingerprint
PACS:
-
DOI:
10.11937/bfyy.20222865
Abstract:
The genetic relationship and DNA fingerprint of 42 ornamental pomegranate cultivars were studied by ISSR markers in this study,in order to provide reference for breeding and utilization of ornamental pomegranate.The results showed that a total of 327 bands were amplified from 16 ISSR primers with good polymorphism，of which 279 were polymorphic loci.The average polymorphic ratio of the primers was 85.32%.The genetic similarity coefficient between 42 cultivars was from 0.46 to 0.98 that showed the genetic diversity of 42 ornamental cultivars was relatively rich.The clustering results by UPGMA showed that 42 cultivars could be divided into four groups at GS 0.629.The clustering results by ISSR marker did not accord with the classification results based on morphological method and geographical origin.The cultivar distinguish rate of 16 primers ranged from 16.67% to 88.10%，so 16 single primers could not distinguish all cultivars.The maximum genetic similarity coefficient (GS) of UBC835/UBC827 combination between cultivars was 0.97 and the distinguish efficiency for 42 cultivars was 100.00%.Therefore the DNA bands form primer combination UBC835/UBC827 were used to construct the DNA fingerprint of 42 ornamental pomegranate cultivars.

References:

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Last Update: 2023-09-19