|Table of Contents|

Virus Detections and Fruit Quality Analysis on Micro-propagation Seedlings of Strawberry

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2023年02
Page:
1-9
Research Field:
Publishing date:

Info

Title:
Virus Detections and Fruit Quality Analysis on Micro-propagation Seedlings of Strawberry
Author(s):
WANG Jianhui; XU Rui; GONG Xiaoyuan; LI Xiang; LIU Dayu; ZHANG Yin
(School of Food and Biological Engineering，Chengdu University，Chengdu,Sichuan 610106)
Keywords:
Fragaria × ananassa; Strawberry mild yellow edge virus; real-time quantitative PCR; meristem tissues culture
PACS:
-
DOI:
10.11937/bfyy.20221914
Abstract:
Taking two types of seedlings using large or small sizes explants by meristem tissues culture,obtaining regenerated plantlets were detected for Strawberry mild yellow edge virus.The virus-free mother plants and virus-undetermined control were propagated to produce daughter plants using stolon.Then virus incidences and fruit quality between two kinds of daughter plants were investigated,respectively,in order to provide reference for the virus-free seedlings application in future.The virus elimination studies would be useful for virus-free seedlings application in future.The results showed that virus concentration differences in plants were found between different cultivars，and virus coat protein expression was consistent with its transcription level.Meristem tissues using explant in size of 5 mm in stolon were induced to adventitious buds and then differentiated to in vitro plantlets at the first round of virus elimination.However，a few in vitro plantlets regenerated from explant in size of 5 mm were still infected by lower virus.In comparison，regeneration ratio at the second round of meristem tissues culture using explant in size of 0.5 mm was only 31%.Besides，all seedlings regenerated from the second of meristem culture were found virus free.Two types of daughter plants propagated using stolon from virus-free mother plants and virus-undetermined control were planted at an experimental plot，respectively.The SMYEV incidences were significantly different between two types of daughter plants.The titratable acidity was significantly lower in virus-free daughter plants than control.In conclusion，the virus-free mother plants were successfully produced by meristem tissues culture using explants in sized of 0.5 mm，which could benefit for strawberry propagation program in future.

References:

[1]DIENGNGAN S,MAHADEVAMMA M,MURTHY B N，et al.Efficacy of in vitro propagation and crown sizes on the performance of strawberry (Fragaria × ananassa Duch.) cv.Festival under field condition[J].Journal of Agricultural Science and Technology,2016,18(1）：255-264.[2]MARTIN R R，TZANETAKIS I E.Characterisation and recent advances in detection of strawberry viruses[J].Plant Disease，2006，90:384-396.[3]ROJAS P，ALMADA R D，SANDOVAL C，et al.Occurrence of aphidborne viruses in southernmost South American populations of Fragaria chiloensis ssp，Chiloensis[J].Plant Pathol，2012，62：428-435.[4]TAKAMURA Y，YAMARYO S，WANG W Q，et al.First report of the complete genomic sequences of strawberry mild yellow edge virus and strawberry vein banding virus isolated in Japan[J].Journal of General Plant Pathology，2020，86:503-506.[5]LUCIANI C，CELLI M G，TORRICO A K，et al.Incidence and prevalence of aphid-borne viruses infecting strawberry in Argentina[J].Ann Appl Biol，2018，173:80-91.[6]HEPP R F，MARTIN R R.Occurrence of strawberry mild yellow-edge associated virus in wild Fragaria chiloensis in South America[C].XV International Symposium on Small Fruit Virus Diseases 308,1991:57-60.[7]MARTIN R R，CONSTABLE F，TZANETAKIS I E.Quarantine regulations and the impact of modern detection methods[J].Annu Rev Phytopathol，2016，54：189-205.[8]POREBSKI S，BAILEY LG，BAUM B R.Modification of a CTAB DNA extraction protocol for plants containing high polysaccharide and polyphenol components[J].Plant Mol Biol Rep，1997（15）：8-15[9]WEI T，LU G J，CLOVER G.Novel approaches to mitigate primer interaction and eliminate inhibitors in multiplex PCR，demonstrated using an assay for detection of three strawberry viruses[J].J Virol Methods，2008，151：132-139.[10]VEETIL T T，HO T，MOYER C，et al.Detection of Strawberry necrotic shock virus using conventional and TaqMan quantitative RT-PCR[J].Journal of Virological Methods，2016，235：176-181.[11]DONNOLI R，SUNSERI F，MARTELLI G，et al.Somatic embryogenesis，plant regeneration and transformation in Fragaria spp[J].Acta Hortic，2001，560：235-239.[12]KIKAS A，LIBEK A，VASAR V.Influence of micropropagation on the production of strawberry runner plants，yield and quality[J].Acta Hortic,2006，708:241-244.[13]SANSAVINI S，ROSATI P，GAGGIOLI D，et al.Inheritance and stability of somaclonal variations in micropropagated strawberry[J].Acta Hortic，1990，280：375-384.[14]WANG J H,LIU J J,CHEN K L,et al.Anthocyanin biosynthesis regulation in the fruit of Citrus sinensis cv.Tarocco[J].Plant Mol Biol Rep,2016(34):1043-1055.[15]THOMPSON J R，JELKMANN W.Strain diversity and conserved genome elements in strawberry mild yellow edge virus[J].Arch Virol，2004，149：1897-1909.[16]TORRICO A K，CELLI M G，CAFRUNE E E，et al.Genetic variability and recombination analysis of the coat protein gene of strawberry mild yellow edge virus[J].Australas Plant Path，2016，45：401-409.[17]JAAG H M，NAGY P D.The combined effect of environmental and host factors on the emergence of viral RNA recombinants[J].PLoS Pathog，2010，6(10)：e1001156.[18]TORRICO A K，SALAZAR S M，KIRSCHBAUM D S，et al.Yield losses of asymptomatic strawberry plants infected with strawberry mild yellow edge virus[J].Eur J Plant Pathol，2017，150(4):983-990.[19]XIANG Y，NIE X Z，BERNARDY M，et al.Genetic diversity of strawberry mild yellow edge virus from eastern Canada[J].Archives of Virology，2020,165:923-935.[20]TZANETAKIS I E，MARTIN R R.A systems-based approach to manage strawberry virus diseases[J].Can J Plant Pathol，2017，39(1)：5-10.[21]WHITEHOUSE A B，GOVAN C L，HAMMOND K J，et al.Meristem culture for the elimination of thestrawberry crown rot pathogen Phytophthora Cactorum[J].Journal of Berry Research，2011，1(3):129-136.[22]CHEONG E J，MOCK R，LI R H.Elimination of five viruses from sugarcane using in vitro cultureof axillary buds and apical meristems[J].Plant Cell Tiss Org，2012，109：439-445[23]张志宏,肖敏,杨洪一,等.草莓病毒脱除方法的比较与评价[J].果树学报,2006(5):720-723.[24]邹小花,杨海云,况慧云,等.不同栽培模式下草莓脱毒苗与常规苗对生长和结果影响的比较[J].北方园艺,2020(5)：39-40.[25]王峰.草莓脱毒苗与常规苗田间性状比较试验[J].山西果树,2016(2):10-11.

Memo

Memo:
-
Last Update: 2023-02-08