|Table of Contents|

Cloning and Bioinformatics of Cherry green ring mottle virus Coat Protein Gene

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2022年15
Page:
10-15
Research Field:
Publishing date:

Info

Title:
Cloning and Bioinformatics of Cherry green ring mottle virus Coat Protein Gene
Author(s):
YANG Yang1; GAO Chenmeng1; ZHAO Zhen1; CAO Xinran2; LI Qingliang1; WANG Deya1
(1.College of Life Sciences，Zaozhuang University，Zaozhuang,Shandong 277160；2.College of Plant Health and Medicine，Qingdao Agricultural University，Qingdao,Shandong 266109)
Keywords:
Cherry green ring mottle virus; coat protein gene; sequence analysis
PACS:
-
DOI:
10.11937/bfyy.20215128
Abstract:
The sweet cherry leaves collected from Yantai，which were infected by Cherry green ring mottle virus (CGRMV)，were used as experimental material.And the nucleic acid sequence，evolutionary relationship，protein properties and structure of CGRMV-CP were analyzed by means of RT-PCR and bioinformatic analysis，in order to provide reference for further functional research and detection applications.The results showed that the coat protein gene was determined to be 807 bp (MW201963)，which encoded a 29.71 kD protein composed of 268 amino acid residues.Compared with 17 reported sequence showed that，the coincidence rate of nucleotide sequences was 75.84%-96.28%，and the amino acid sequence was 81.72%-98.51%.And the CGRMV-CP was a hydrophobic non-transmembrane protein with isoelectric point pI of 8.25，consisting of 6 α-helices，7 β-sheet structure.

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Last Update: 2022-09-08