|Table of Contents|

Optimization of Extraction Technology of Total Flavonoids and Its Antioxidant Capacity From Aerial Part of Shexian Bupleurum

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2022年06
Page:
92-98
Research Field:
Publishing date:

Info

Title:
Optimization of Extraction Technology of Total Flavonoids and Its Antioxidant Capacity From Aerial Part of Shexian Bupleurum
Author(s):
YE Jia1; 2; LI Jianfei3; CHEN Jiangkui1; 2; SHI Mingli1; YANG Xiangyu1; 2; GUO Haiyan1; 2
(1.College of Life Science and Engineering，Handan University，Handan，Hebei 056005；2.Wild Plant Resources Research Centre in Taihang Mountain of Southern Hebei Province，Handan，Hebei 056005；3.Shexian Qingyang Seed Industry Co.Ltd.，Handan,Hebei 056400)
Keywords:
aerial part of bupleurum; total flavonoids; optimization; antioxidant activity
PACS:
-
DOI:
10.11937/bfyy.20214464
Abstract:
Taking the aerial part of Shexian bupleurum as test materials,the ethanol reflux extraction process of total flavonoids was studied and optimized by single factors test and four factors and three-levels orthogonal test.The antioxidant activity of the extract and compound with vitamin C were studied,in order to provide reference for the further development and utilization of bupleurum in Shexian.The results showed that,1) the best extraction process for total flavonoids from aerial part of bupleurum was solid-liquid ratio was 1∶20 g?mL-1，extraction time was 2 hours，extraction temperature was 70 ℃，and ethanol concentration was 60%.2) The half inhibition rate concentration (IC50) of total flavonoids from aerial part of bupleurum against DPPH?，?OH and O?2 radical were 0.223，0.413 mg?mL-1 and 0.445 mg?mL-1，respectively.3) It showed a certain synergistic effect after compounding with vitamin C，the best synergistic effect was compound solution (1∶3) in all experimental groups.In conclusion,the total flavonoids in the aerial part of Shexian bupleurum had good antioxidant capacity.

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Last Update: 2022-05-25