|Table of Contents|

Effects of Different Ammonium Nitrogen Ratios on Growth and Ammonium Assimilation of Tomato

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2022年03
Page:
9-17
Research Field:
Publishing date:

Info

Title:
Effects of Different Ammonium Nitrogen Ratios on Growth and Ammonium Assimilation of Tomato
Author(s):
XUN Zhili1; 2; GAO Jingjing1; LI Yaling1; WEN Xiangzhen1
(1.College of Horticulture,Shanxi Agricultural University,Taigu,Shanxi 030801;2.Pomology Institute,Shanxi Agricultural University,Taiyuan,Shanxi 030032)
Keywords:
ammonium nitrogen; biomass; ammonium assimilation; growth inhibition
PACS:
-
DOI:
10.11937/bfyy.20213104
Abstract:
Tomato ‘Kuiguanhongfeng’ was used as the experimental material，perlite/vermiculite (V/V 2∶1) was used as the matrix，and ammonium nitrogen (0 mmol·L-1)+nitrate nitrogen (15 mmol·L-1) (A0N15，control)，5 mmol·L-1+10 mmol·L-1 (A5N10)，7.5 mmol·L-1+7.5 mmol·L-1 (A7.5N7.5)，10 mmol·L-1+5 mmol·L-1 (A10N5)，15 mmol·L-1+0 mmol·L-1 (A15N0)，were designed.The aim of the study to explore the effect of different ratios from ammonium nitrogen and nitrate nitrogen on growth and ammonium assimilation of tomato under the same nitrogen level,in order to provide a reference for realizing the high efficiency ammonium assimilation.The results showed that compared with the control，the total dry weight were increased by 27% and 20% in A5N10 and A7.5N7.5 treatment，was no significant difference in A10N5 treatment，but was decreased by 22% in A15N0 treatment.It was found that the content of total free amino acids in roots and leaves were increased by more than 40% with the increase of ammonium nitrogen ratio.The activities of glutamine synthetase (GS)，glutamate synthetase (GOGAT) and glutamate dehydrogenase (GDH) involved in nitrogen metabolism in roots were significantly higher than those in control.At the same time，the contents of glutamine (Gln) and glutamic acid (Glu) were increased with the increase of ammonium nitrogen ratio，but the contents of Glu were decreased significantly with the treatment of A10N5 and A15N0，and the contents of soluble sugar，starch and chlorophyll were decreased.It was concluded that when the concentration of ammonium nitrogen in nutrient solution reached 10 mmol·L-1 or above，the plant growth was inhibited，which may be due to the insufficient carbon assimilation caused by the decreased of chlorophyll content in leaves，which due to the inhibition of nitrogen assimilation caused by the decrease of Glu content，which inhibited the plant growth and reduced the biomass.

References:

[1]MILLER A J，CRAMER M D.Root nitrogen acquisition and assimilation[J].Plant Soil，2005，274：1-36.[2]LUO J，LI H，LIU T，et al.Nitrogen metabolism of two contrasting poplar species during acclimation to limiting nitrogen availability[J].J Exp Bot，2013，64：4207-4224.[3]张英鹏，徐旭军，林咸永，等.氮素形态对菠菜可食部分硝酸盐和草酸累积的影响[J].植物营养与肥料学报，2006(2)：2227-2232.[4]孙菲菲，李英，侯喜林，等.硝态氮对不结球白菜产量与主要营养品质及硝酸盐含量的影响[J].南京农业大学学报，2008(2)：37-40.[5]FUERTES-MENGIZABAL T，GONZALEZ-TORRALBA J，ARREGUI L M，et al.Ammonium as sole N source improves grain quality in wheat[J].Sci Food Agric，2013，93：2162-2171.[6]GAO Y，LI Y，Yang X，et al.Ammonium nutrition increases water absorption in rice seedlings (Oryza sativa L.) under water stress[J].Plant and Soil，2010，331：193-201.[7]BLOOM A J，BURGER M，RUBIO-ASENSIO J S，et al.Carbon dioxide enrichment inhibits nitrate assimilation in wheat and Arabidopsis[J].Science，2010，328：899-903.[8]VEGA-MAS I，CARMEN M，PE′REZ-DELGADO，et al.Plant nitrogen assimilation and its regulation：A complex puzzle with missing pieces[J].Current Opinion in Plant Biology，2015(25)：115-122.[9]FERNANDEZ-CRESPO E，SCALSCHI，LLORENS E，et al.NH+4 protects tomato plants against Pseudomonas syringae by activation of systemic acquired acclimation[J].J Exp Bot，2015，66(21)：6777-6790.[10]XUN Z L，GUO X F，LI Y L，et al.Quantitative proteomics analysis of tomato growth inhibition by ammonium nitrogen[J].Plant Physiology and Biochemistry，2020,154：129-141.[11]CATALDO D A，MAROON M，SCHRADER L E，et al.Rapid colorimetric determination of nitrate in plant tissue by nitration of salicylic acid[J].Commun Soil Sci Plant Anal,1975(6)：71-80.[12]SOLORZANO L.Determination of ammonia in natural waters by the phenolhy-pochlorite method[J].Limnol Oceanogr,1969(14)：799-801.[13]ZHANG Y，HU X H，SHI Y，et al.Beneficial role of exogenous spermidine on nitrogen metabolism in tomato seedlings exposed to saline-alkaline stress[J].J Am Hortic Sci，2013，138：38-49.[14]VEGA-MAS I，PE′REZ-DELGADO C M，Marino D，et al.Elevated CO2 induces root defensive mechanisms in tomato plants when dealing with ammonium toxicity[J].Plant Cell Physiol，2017，0：1-14.[15]BRADFORD M M.A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding[J].Anal Biochem，1976，72：248-254.[16]DATTA R，SHARMA R.Temporal and spatial regulation of nitrate reductase and nitrite reductase in greening maize leaves[J].Plant Sci,1999，144：77-83.[17]LIN C C，KAO C H.Disturbed ammonium assimilation is associated with growth inhibition of roots in rice seedlings caused by NaCl[J].Plant Growth Regul,1996（18）：233-238.[18]MEI H S，THIMANN K V.The relation between nitrogen deficiency and leaf senescence[J].Physiol Plant，1984，62:157-161.[19]徐新娟，李庆余，孙瑞，等.不同形态氮素对樱桃番茄果实发育和品质的影响[J].植物营养与肥料学报，2009(6)：1425-1432.[20]LIU G Y，DU Q J，LI J M.Interactive effects of nitrate-ammonium ratios and temperatures on growth，photosynthesis，and nitrogen metabolism of tomato seedlings[J].Sci Horticult，2015，214：41-50.[21]公华锐，骆洪义，亓艳艳，等.不同硝铵比对基质栽培番茄氮素代谢关键酶及其氮素利用效率的影响[J].北方园艺，2017(24)：7-16.[22]毕兆东，王世金，程青青，等.不同氮肥及配比对韭菜产量和品质的影响[J].东北农业大学学报，2010，41(11)：37-41.[23]ALEXANDER K G，MILLER M H，BEACCHAMP E G.The effect of an NH+4-enhanced nitrogen source on the growth and yield of hydroponically grown maize (Zea mays L.)[J].J Plant Nutr，1991(14)：31-34.[24]TAKA′CS E,TE′CSI L.Effects of NO-3/NH+4 ration on photosynthetic rate，nitrate reductase activity and chloroplast ultrastructure in three cultivars of red pepper (Capsicum annuum L.)[J].J Plant Physiol，1992，140：298-305.[25]李娟，周健民.水培条件下CO2与NH+4/NO-3配比对番茄幼苗生育的影响[J].农业工程学报，2006(7）：138-142.[26]MASCLAUX-DAUBRESSE C，REISDORF-CREN M，PAGEAU K，et al.Glutamine synthetase-glutamate synthase pathway and glutamate dehydrogenase play distinct roles in the sink-source nitrogen cycle in tobacco[J].Plant Physiol,2006，140：444-456.[27]MIYASHITA Y，GOOD A G.Contribution of the GABA shunt to hypoxia-induced alanine accumulation in roots of Arabidopsis thaliana[J].Plant Cell Physiol,2008，49：92-102.[28]TAKACCS E，TECCSI L.Effects of NO-3/NH+4 ration on photosynthetic rate，nitrate reductase activity and chloroplast ultrastructure in three cultivars of red pepper (Capsicum annuum L.)[J].Journal of Plant Physiology，1992，140：298-305.[29]张宏纪,马凤鸣,李文华,等.不同形态氮素对甜菜谷氨酰胺合成酶活性的影响[J].黑龙江农业科学，2001(6)：7-10.[30]LIU Y，VON WIRE′N N.Ammonium as a signal for physiological and morphological responses in plants[J].J Exp Bot，2017，63，3777-3788.[31]戴廷波，曹卫星，孙传范，等.增铵营养对小麦光合作用及硝酸还原酶和谷氨酰胺合成酶的影[J].应用生态学报，2003(9)：1529-1532.[32]KONISHI N，ISHIYAMA K，MATSUOKA K，et al.NADH-dependent glutamate synthase plays a crucial role in assimilating ammonium in the Arabidopsis root[J].Physiol Plant，2014，152：138-151.[33]LEA P J，MIFLIN B J.Glutamate synthase and the synthesis of glutamate in plants[J].Plant Physiol Bioch，2003，41：555-564.[34]BIALCZYK J，LECHOWSKI Z，DZIGA D，et al.Carbohydrate and free amino acid contents in tomato plants grown in media with bicarbonate and nitrate or ammonium[J].Acta Physiol Plant，2005(27)：523-529.[35]GAUFICHON L，REISDORF-CREN M，ROTHSTEIN S J，et al.Biological functions of asparagine synthetase in plants[J].Plant Sci，2010，179：141-153.

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Last Update: 2022-04-09