|Table of Contents|

Preliminary Study on the Mechanism and Induction Effects of Exogenous MeJA on Chinese Cabbage Under the Stress of Clubroot

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2022年03
Page:
1-8
Research Field:
Publishing date:

Info

Title:
Preliminary Study on the Mechanism and Induction Effects of Exogenous MeJA on Chinese Cabbage Under the Stress of Clubroot
Author(s):
WANG Ya; XIAO Chen; DONG Jun; LI Huinan; ZHAO Limin
(College of Horticulture，Northwest A&F University，Yangling，Shaanxi 712100)
Keywords:
MeJA; Chinese cabbage; Plasmodiophora brassicae; induced resistance
PACS:
-
DOI:
10.11937/bfyy.20213122
Abstract:
Taking Chinese cabbage ‘Jinguan’ as the test material，the different concentrations (50-250 μmol·L-1) of MeJA were applied to the roots of Chinese cabbage under clubroot stress，the effects of MeJA on seedlings growth，defense enzymes，osmoregulation substances and related defense gene expression were studied，in order to provide reference basis for the prevention and treatment of Chinese cabbage clubroot by exogenous MeJA.The results showed that exogenous MeJA could significantly reduce the disease index which was negatively correlated with MeJA concentration，and also significantly decrease MDA and osmoregulation substances contents，especially at the concentration of 100 μmol·L-1.While，the growth of cabbage seedlings was inhibited when applying more than 150 μmol·L-1 MeJA.Additionally，exogenous MeJA application increased chlorophyll content and antioxidant enzyme activity in Chinese cabbage leaves，as well as up-regulated disease-related protein genes [STBX]PR-1 and PR-3[STBZ]，so that the resistance of Chinese cabbage seedlings to clubroot was improved.Through comprehensive analysis，application of 100 μmol·L-1 MeJA could effectively promote the resistance of Chinese cabbage seedlings to clubroot most.

References:

[1]周燚,杨廷宪,赵毓潮,等.大白菜根肿病综合防治新技术研究[J].中国蔬菜,2012(2):83-86.[2]王妮妍,蒋德安.茉莉酸及其甲酯与植物诱导抗病性[J].植物生理学通讯,2002(3):279-284.[3]何红卫,廖令洁,肖文娟,等.茉莉酸甲酯对烟草幼苗抗病毒相关酶活性的影响[J].暨南大学学报(自然科学与医学版),2005(3):406-411.[4]牛吉山,刘靖,马文斌,等.茉莉酸甲酯诱导的小麦白粉病抗性与9个抗病相关基因表达间的关系(英文)[J].Agricultural Science & Technology,2011,12(4):504-508.[5]VIJAYAN P，SHOCKEY J，LEVESQUE C A，et al.A role for jasmonate in pathogen defense of Arabidopsis[J].Plant Biol，1998,95(12)：7209-7214[6]许倩倩.水杨酸（SA）和苯并噻二唑（BTH）对甘蓝根肿病抗性相关生理指标的影响[D].重庆:西南大学,2015.[7]王新月.太白高山地区十字花科蔬菜根肿病菌生理小种鉴定及品种抗性筛选[D].杨凌:西北农林科技大学,2017.[8]朱红芳,高倩倩,李晓锋,等.根肿病胁迫下外源SA对不结球白菜抗性诱导和生理生化的影响[J].西北植物学报,2017,37(2):297-306.[9]高俊凤.植物生理学实验指导[M]．北京:高等教育出版社,2006.[10]李合生.植物生理生化实验原理和技术[M].北京:高等教出版社,2006.[11]韩之琪.氟唑活化酯诱导大白菜抗根肿病机制的研究[D].北京:中国农业科学院,2015.[12]HUSHNA H A,KIYOHARA D,KIMURA M，et al.Systemic resistance induced by volatile organic compounds emitted by plant growth-promoting fungi in Arabidopsis thaliana[J].PLoS One,2014,9(1):e86882.[13]伍文宪,刘勇,张蕾,等.芸薹根肿菌致病机理的研究进展[J].中国农学通报,2015,31(18):260-265.[14]张浩.外源茉莉酸甲酯、犬尿氨酸和氨基氧乙酸对大白菜BrCYP79B2/B3表达及相关生理生化指标的影响[D].杭州:浙江农林大学,2015.[15]李慧楠,董军,王雅,等.抗根肿病大白菜品种抗性鉴定与性状评价[J].中国瓜菜,2020,33(7):39-43.[16]贾凯,闫会转,高杰.外源MeJA对芜菁幼苗生长及抗氧化酶活性的影响[J].新疆农业科学,2020,57(5):806-813.[17]滑莎,继丹,刘欣,等．外源信号物质.MeJA诱导糖槭叶部营养物质含量的变化[J].林业科技,2018,43(1):9-12.[18]牛吉山,刘靖,倪永静,等.茉莉酸对〖STBX〗PR-1、PR-2、PR-5和Ta-JA2〖STBZ〗基因表达以及小麦白粉病抗性的诱导[J].植物病理学报,2011,41(3):270-277.[19]王文艳,岳林许,张演义,等.葡萄SA和JA信号转导重要基因克隆及其对外源信号应答分析[J].园艺学报,2012,39(5):817-827.[20]COHEN Y.Local and systemic protection against Phytophthora infestans induced in potato and tomato plants by jasmonic acid and jasmonic methylester[J].Phytopathology,1993，83(10)：1054-1062.

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Last Update: 2022-04-09