|Table of Contents|

Cloning and Expression Analysis of FaABI5 Gene in Strawberry

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2022年01
Page:
31-39
Research Field:
Publishing date:

Info

Title:
Cloning and Expression Analysis of FaABI5 Gene in Strawberry
Author(s):
LI Jiale; DENG Lili; XU Shiqiong; CHENG Yuxing; LIU Yongqiang; TANG Haoru
(College of Horticulture,Sichuan Agricultural University,Chengdu,Sichuan 611130)
Keywords:
strawberry; FaABI5 gene; clone; expression pattern
PACS:
-
DOI:
10.11937/bfyy.20212123
Abstract:
The FaABI5 gene was cloned from the cultivated strawberry ‘Benihoppe’ by homologous cloning,the expression pattern of FaABI5 was obtained by qRT-PCR,the protein was characterized by transcriptional activity and subcellular localization,and the protein was induced to expressed using Escherichia coli,in order to provide a reference for further elucidation of the gene function of FaABI5.The results showed that the open reading frame of strawberry FaABI5 gene was 1 329 bp,encoding 442 amino acids,with a predicted molecular weight of about 47.1 kDa and a theoretical isoelectric point of 9.70,which was an unstable hydrophilic protein.Amino acid sequence comparison showed that this proteins had high identity with ABI5 proteins of other species,and the phylogenetic tree indicated that strawberry FaABI5 clustered with homologous proteins in wild strawberry and Chinese rose into one branch.A 1 500 bp long promoter sequence upstream of the start codon of this gene was obtained.In addition to a large number of ABA response elements ABRE,the promoter of FaABI5 contains many light,phytohormone and abiotic stress response elements.qRT-PCR results showed that FaABI5 was most highly expressed in roots and stems of strawberry,and less expressed in fruits.In yeast,FaABI5 does not have transcriptional activation ability.Subcellular localization experiments demonstrated that FaABI5 was a nuclear localization protein.Bioactive FaABI5 recombinant protein could be obtained by self-induction at 28 ℃ for 24 hours in E.coli Rosetta (DE3).

References:

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Last Update: 2022-04-01