|Table of Contents|

Phyllosphere Bacterial Community Composition on Phaseolus vulgaris Linn.Under Different Nitrogen and Potassium Levels

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2022年04
Page:
76-84
Research Field:
Publishing date:

Info

Title:
Phyllosphere Bacterial Community Composition on Phaseolus vulgaris Linn.Under Different Nitrogen and Potassium Levels
Author(s):
DING Lu1; CUI Lixing1; NIU Yingchao1; DAI Xiaohua1; 2 GUO Qingyun1
(1.School of Life Sciences,Gannan Normal University,Ganzhou,Jiangxi 341000;2.National Navel-orange Engineering Research Center,Ganzhou,Jiangxi 341000)
Keywords:
Phaseolus vulgaris Linn.; nitrogen and potassium applications; phyllosphere bacterial diversity; high-throughput sequencing
PACS:
-
DOI:
10.11937/bfyy.20212058
Abstract:
Taking Phaseolus vulgaris Linn.as test material,the bacterial OTUs,diversity index and species identification on leaf surfaces of Phaseolus vulgaris Linn.were investigated in greenhouse under different nitrogen and potassium application using high-throughput sequencing method based on 16S rRNA hypervariable region V3-V4,in order to provide a reference for the study on precision fertilization and plant-microorganism relationship.The results showed that the bacterial OTUs number on Phaseolus vulgaris Linn.phyllosphere significantly decreased under high and deficiency nitrogen,high potassium,while the OTUs number increased under potassium deficiency.Alpha diversity analysis showed that the richness and diversity of bacterial community obviously increased under normal nitrogen and potassium deficiency,and significantly decreased under excessive nitrogen and potassium fertilization,or nitrogen deficiency.The phyllosphere bacterial community showed the dominant phyla were Proteobacteria,Firmicutes,Bacteroidetes and Actinobacteria.Excessive and lack of nitrogen and potassium could lead to the overgrowth of Escherichia coli and Bacteroides,and interfere or inhibit the growth Burkholderia and Bacillus,which could affect the ability of nitrogen fixation and disease resistance of plants,and then slowing down the growth and yield of crops.

References:

[1]LINDOW S E,BRANDL M T.Microbiology of the phyllosphere[J].Applied Environmental Microbiology,2003,69(4):1875-1883.[2]VORHOLT J A.Microbial life in the phyllosphere[J].Nature Reviews Microbiology,2012,10(12):828-840.[3]PEUELAS J,TERRADAS J.The foliar microbiome[J].Trends Plant Science,2014,19(5):278-280.[4]潘建刚,呼庆,齐鸿雁,等.叶际微生物研究进展[J].生态学报,2011,31(2):583-592.[5]高爽,刘笑尘,董铮,等.叶际微生物及其与外界互作的研究进展[J].植物科学学报,2016,34(4):654-661.[6]卢昕,黄贵修.植物内生细菌生防作用研究进展[J].华南热带农业大学学报,2007,13(4):28-33.[7]孙泓,李慧,詹亚光,等.不同生境中桂花和夹竹桃叶际细菌的群落结构[J].应用生态学报,2018,29(5):1653-1659.[8]吴林坤,陈军,杨波,等.地黄连作对叶际细菌群落结构及多样性的影响[J].应用生态学报,2019,30(10):3509-3517.[9]罗路云,张卓,金德才,等.南瓜白粉病不同病情等级下叶际细菌群落结构和多样性[J].植物病理学报,2017,47(5):688-695.[10]刘利玲,李会琳,蒙振思,等.青杨雌雄株叶际微生物群落多样性和结构的差异[J].微生物学报,2020,60(3):556-569.[11]冯国军,刘大军.菜豆的营养价值评价与分析[J].北方园艺,2016(24):200-208.[12]张慎好,武春成,冯志红,等.冲施肥对菜豆产量效应的影响[J].安徽农业科学,2009,37(11):4947-4949.[13]何永梅,欧迎峰,徐丽红,等.菜豆高产栽培科学施肥技术[J].科学种养,2021(1):36-38.[14]MIZRAHI-MAN O,DAVENPORT E R,GILAD Y.Taxonomic classification of bacterial 16S rRNA genes using short sequencing reads:Evaluation of effective study designs[J].PLoS One,2013,8(1):e53608.[15]EVAN B,RAM R J,DILLON M R,et al.Reproducible,interactive,scalable and extensible microbiome data science using QIIME 2[J].Nature Biotechnology,2019,37(8):852-857.[16]CALLAHAN B J,MCMURDIE P J,ROSEN M J,et al.DADA2:High-resolution sample inference from Illumina amplicon data[J].Nature Methods,2016,13(7):581-583.[17]ROGNES T,FLOURI T,NICHOLS B,et al.VSEARCH:A versatile open source tool for metagenomics[J].PeerJ,2016,4(10):e2584.[18]杨蕾,杨海健,李勋兰,等.滋生青苔对柑橘叶际生物多样性的影响[J].植物保护,2019,45(6):98-105,123.[19]贺纪正,李晶,郑袁明.土壤生态系统微生物多样性-稳定性关系的思考[J].生物多样性,2013,21(4):411-420.[20]COPELAND J K,YUAN L,LAYEGHIFARD M,et al.Seasonal community succession of the phyllosphere microbiome[J].Molecular Plant-microbe Interactions,2015,28(3):274-285.[21]刘玮,邓光华,张嘉超,等.不同施肥处理对毛竹根际微生物影响及其PCR-DGGE分析[J].东北林业大学学报,2011,39(5):50-53.[22]康利允,常高正,马政华,等.不同氮钾肥用量对甜瓜坐果节位叶片生理特性的影响[J].中国土壤与肥料,2019(4):96-104.[23]李静文,刘晓颖,李士坤,等.氮磷钾配比施肥对毛竹出笋及叶片生理特性的影响[J].西南农业学报,2020,33(12):2885-2890.[24]林标声,宋昭昭,张丽丽,等.巨菌草不同生长时期根,茎,叶内生细菌组成的多样性及差异[J].福建农林大学学报,2018,47(3):352-360.[25]朱玥妍,刘姣,杜春梅.芽孢杆菌生物防治植物病害研究进展[J].安徽农业科学,2012,40(34):16635-16638.

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Last Update: 2022-05-19