|Table of Contents|

Establishment of Rapid Propagation System for Tissue Culture of Two Male Sterile Lilies

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2021年20
Page:
67-76
Research Field:
Publishing date:

Info

Title:
Establishment of Rapid Propagation System for Tissue Culture of Two Male Sterile Lilies
Author(s):
HAN Songming; MENG Qingxue; WU Yanfei; YAN Kaili; HU Wan; FAN Jinping
(College of Horticulture and Landscape Architecture,Northeast Agricultural University,Harbin,Heilongjiang 150030)
Keywords:
lily; male sterility; tissue culture; rapid propagation
PACS:
-
DOI:
10.11937/bfyy.20211452
Abstract:
Taking two kinds of male sterile lilies ‘5-4’ and ‘5-35’ with good color,flower type and excellent growth as test materials,tissue culture methods were used to study different disinfection times,hormone concentrations and different substrate formulation on the tissue culture process of two kinds of male sterile lilies,in order to establish their rapid propagation system and provide reference for mass production of non-pollen lilies.The results showed that the best way to sterilize explants was 75% ethanol disinfection for 20 seconds,10% NaClO solution for 8 minutes,and 75% ethanol for 30 seconds,10% NaClO solution for 6 minutes；the best induction medium was MS+NAA 1.0 mg?L-1+6-BA 0.5 mg?L-1 and MS+NAA 1.5 mg?L-1+6-BA 0.5 mg?L-1；the best proliferation medium was MS+6-BA 1.5 mg?L-1+NAA 0.5 mg?L-1；the best rooting medium was 1/2MS+IBA 0.1 mg?L-1+NAA 0.1 mg?L-1+sucrose 40 g?L-1 and 1/2MS+IBA 0.2 mg?L-1+NAA 0.1 mg?L-1+sucrose 40 g?L-1；the best transplanting substrate ratio was vermiculite∶perlite∶peat=1∶1∶1.

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Last Update: 2022-02-07