|Table of Contents|

Effects of Liquid Organic Fertilizer Partial Replacement of Chemical Fertilizer on Fungal Flora in Tomato Root Zone in Greenhouse

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2021年12
Page:
79-87
Research Field:
Publishing date:

Info

Title:
Effects of Liquid Organic Fertilizer Partial Replacement of Chemical Fertilizer on Fungal Flora in Tomato Root Zone in Greenhouse
Author(s):
XIA Xiubo; LI Tao; YAO Jiangang; CAO Shoujun; ZHANG Lili; WANG Hongyun
(Yantai Academy of Agricultural Sciences，Yantai，Shandong 265500)
Keywords:
greenhouse tomato; liquid organic fertilizer; chemical flushing fertilization; fungi; diversity
PACS:
-
DOI:
10.11937/bfyy.20203621
Abstract:
Taking ‘Yanfen 209’ tomato as test material,high-throughput sequencing technology was used to analyze the effects of liquid organic fertilizer partial replacement of chemical flushing fertilization on the diversity,structural composition and differences of fungal community in tomato root zone soil samples,in order to provide a reference basis for reasonable fertilization of protected tomato production.The results showed that,at 97% similarity level,301 OTU,8 phylum,20 classes,41 orders,78 families,118 genus and 159 species were found.There were 27 total OTU values in the three treatments and the control samples,and there were 106,34 and 16 OTU values in the three treatments,respectively.It was the highest diversity and richness of fungi in T_ R30 treatment soil sample.The dominant phylum were Basidiomycota,Ascmycota and Mortierellomycota.The dominant genus were unclassifiled_f_Ceratobasidiaceae,Mortierella and Mycothermus.Through cluster analysis,principal component analysis and non metric multi-dimensional scale analysis,it was found that there were high similarity of fungal colony composition and greatest commonness between the control sample and T_ R30 treatment sample.The comprehensive analysis showed that liquid organic fertilizer instead of chemical flushing fertilization could increase the species of exogenous fungi in root zone soil,and change the richness,diversity and colony composition,and T_ R30 treatment has obvious advantages.

References:

[1]张福锁.科学认识化肥的作用[J].中国农技推广,2017,33(1):16-19.[2]杨吉祥,马平平.浅谈农药化肥的负面作用及对策[J].中国园艺文摘,2009(6)：158-160.[3]张迎春,颉建明,李静.生物有机肥部分替代化肥对莴笋及土壤理化性质和微生物的影响[J].水土保持学报,2019,33(4):196-205.[4]姜利红,谢桂先,刘强,等.有机无机肥配施对双季稻田土壤微生物和碳库的影响[J].湖南农业大学学报(自然科学版),2018,44(3):295-300.[5]祝英,王治业,彭轶楠,等.有机肥替代部分化肥对土壤肥力和微生物特征的影响[J].土壤通报,2015,46(5):1161-1167.[6]李杰,贾豪语,颉建明,等．生物肥部分替代化肥对花椰菜产量、品质、光合特性及肥料利用率的影响[J].草业学报,2015,24(1):47-55.[7]牛振明,张国斌,刘赵帆,等．生物肥部分替代化肥对甘蓝（Brassica oleracea）养分吸收、光合作用以及品质的影响[J].中国沙漠,2014,34(2):464-471．[8]CHEN S，ZHOU Y，CHEN Y，et al.Fastp：An ultra-fast all-in-one FASTQ preprocessor[J].Bioinformatics,2018,34(17):884-890.[9]MAGO〖AKCˇ〗 T，SALZBERG S L.FLASH：Fast length adjustment of short reads to improve genome assemblies[J].Bioinformatics,2011;27(21):2957-2963.[10]EDGAR R C.UPARSE：Highly accurate OTU sequences from microbial amplicon reads[J].Nat Methods,2013,10(10):996-998.[11]STACKEBRANDT E，GOEBEL B M.Taxonomic note：A place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology[J].International Journal of Systematic Bacteriology，1994，44(4):846-849.[12]WANG Q，GARRITY G M，TIEDJE J M，et al.Naive Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy[J].Appl Environ Microbiol，2007,73(16):5261-5267.[13]赵吉,廖仰南．羊草草地分解者亚系统的特性及作用[J].生态学报,1995,15(4)：359-364．[14]钟文辉,蔡祖聪．土壤微生物多样性研究方法[J].应用生态学报,2004,15(5)：899-904．[15]马利平.生物有机肥替代化肥减施对大豆土壤微生物多样性的影响[D].哈尔滨:黑龙江大学,2019.[16]李宽莹,王泽林,徐兴有,等.不同施肥处理对日光温室内土壤微生物数量与酶活性的影响[J].西北林学院学报,2019,34(2):56-61.[17]王宁,南宏宇,冯克云.化肥减量配施有机肥对棉田土壤微生物生物量、酶活性和棉花产量的影响[J].应用生态学报，2020,31(1)：173-181.[18]白亚丽.日光温室果菜有机肥替代及化肥减施对其生长和土壤环境的影响[D].郑州:河南农业大学,2019.

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Last Update: 2021-09-27