|Table of Contents|

High-density Fermentation Technology of Armillaria mellea YN3862 Strain

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2020年20
Page:
124-129
Research Field:
Publishing date:

Info

Title:
High-density Fermentation Technology of Armillaria mellea YN3862 Strain
Author(s):
LUO Zhiwen1; 2; LIU Yang3; JIAO Yunjian1; 2; DENG Xianyu1; 2; ZHANG Qilin1; 2; LIN Lianbing1; 2
(1.Faculty of Life Science and Technology,Kunming University of Science and Technology,Kunming,Yunnan 650500;2.Engineering Research Center for Replacement Technology of Feed Antibiotics of Yunnan College,Kunming,Yunnan 650500;3.Yunnan Jianengda Pharmaceutical Co.Ltd.,Kunming,Yunnan 650106)
Keywords:
Armillaria mellea; culture medium; liquid fermentation; high-density culture
PACS:
-
DOI:
10.11937/bfyy.20200419
Abstract:
Armillaria mellea YN3862 strain was used as test material, a combination of solid culture and deep liquid fermentation was used to study the optimal mother culture medium and fermentation conditions of A. mellea, in order to achieve the purpose of high-density culture of A. mellea.The results showed that the best culture medium for A.mellea includes sawdust 100 g?L-1,wheat bran 100 g?L-1,glucose 20 g?L-1,agar 15 g?L-1 and beef extract 5 g?L-1.The optimum liquid medium composition was 1% glucose,2% sucrose,3% silkworm pupa powder,0.075% magnesium sulfate heptahydrate,0.15% potassium dihydrogen phosphate and 1% ethanol.Deep liquid fermentation was conducted in a 200 L fermentation tank and filled with 100 L liquid culture medium,the liquid strain was inoculated with 5% inoculation amount,the fermentation temperature was 25 ℃ and the dissolved oxygen was about 55%.At 0-24,24-36,36-48,48-144 hours of fermentation,the rotational speeds were set as 60,80,120,150 r?min-1,respectively.The optimal fermentation time was 120 hours.At this time,the mycelium quantity could reach 2.5 g?(100mL)-1.In conclusion,by using the above medium and deep liquid fermentation technology,the biomass produced was large,and the produced mycelium pellet had good vitality and realized high-density culture.

References:

[1]潘崇环.珍稀食用菌栽培与名贵野生菌的开发利用[M].北京:中国农业出版社,2004.[2]王鑫,亢学平,胡志强,等.蜜环菌原种配方筛选试验[J].中国林副特产,2017(6):41-42.[3]李福后,王伟霞.5株蜜环菌产几种胞外酶活性比较[J].淮海工学院学报(自然科学版),2006,15(3):58-61.[4]吴兴亮,连宾,邹方伦.贵州蜜环菌资源及其生态研究[J].贵州科学,2003,21(3):56-60.[5]NAGAI K,TANAKA J,KIHO T,et al.Synthesis and antitumor activities of mitomycin C (1→3)-β-D-glucan conjugate[J].Chem Pharma Bulletin,1992,40(4):986-989.[6]KIHO T,SHIOSE Y,NAGAI K,et al.Polysaccharides in fungi.XXIX.Structural features of two antitumor polysaccharides from the fruiting bodies of Armillariella tabescens[J].Chem Pharma Bulletin,1992,40(8):2212.[7]OTA Y,INTINI M,HATTORI T.Genetic characterization of heterothallic and non-heterothallic Armillaria mellea sensu stricto[J].Mycological Res,2000,104(9):1046-1054.[8]顾雅君,张明艳,唐宏亮,等.蜜环菌和天麻、猪苓的关系及尚未大量人工驯化原因[J].中国食用菌,2008,27(6):20,28.[9]郭顺星.蜜环菌和猪苓的发育学及其二者的相互作用机理[D].北京:中国协和医科大学,1995.[10]黄靖雯,赖长江生,袁媛,等.猪苓与蜜环菌化学成分研究的相关分析进展[J].中国中药杂志，2017,42(15):2905-2914.[11]唐德华,廖文菊.天麻蜜环菌研究综述[J].安徽农业科学,2015,43(13):31-32.[12]李露,王思爽,马丽颖,等.蜜环菌的活性成分和药用价值[J].吉林医药学院学报,2018,39(4):307-310.[13]OBUCHI T,KONDOH H,WATANABE N,et al.Armillaric acid,a new antibiotic produced by Armillaria mellea[J].Planta Med,1990,56:198-201.[14]王惠国,冯宝民.蜜环菌多糖免疫调节活性的实验研究[J].陕西科技大学学报,2009,27(2):62-64.[15]沈明花,金梅花.密环菌多糖对小鼠急性肝损伤的保护作用[J].食品研究与开发,2014(3):91-93.[16]闫军,王欢,孙冬梅.蜜环菌培养条件优化[J].黑龙江农业科学,2016(3):112-114.[17]陈顺方,祁岑,黄先敏.蜜环菌的生产技术[J].现代农业科技,2009(14):125-127.[18]黄燕芬,唐丽,范成五.蜜环菌原种培养基组分筛选[J].贵州农业科学,2005,33(4):54-55.[19]唐兴国,王伯诚,赖小芳.天麻共生蜜环菌液体种培养基的优化[J].浙江农业科学,2017,58(4):635-637.[20]亢学平,王鑫,胡志强,等.伴栽天麻蜜环菌的适宜培养基筛选[J].食药用菌,2016,24(2):104-105.[21]刘太林,李黎,周正涛,等.六种蜜环菌固体培养基配方的筛选[J].北方园艺,2015（18）:145-148.[22]牛晓静,陈天朝,鲁静,等.槐角中还原糖的含量测定[J].中医药信息,2019(6):18-21.[23]袁媛,刘景圣.蜜环菌液体深层发酵培养基的优选[J].农产品加工(学刊),2009(7):57-59.[24]熊泽,邵伟,乐超银,等.蜜环菌液体发酵条件研究及其饮料开发[J].三峡大学学报(自然科学版),2003,25(6):565-567.[25]邵伟,乐超银,刘世玲,等.蜜环菌深层液体发酵条件研究[J].中国食用菌,2004,23(3):50-51.[26]刘宇,龚思慧,彭楠,等.蜜环菌液态发酵培养基及补料分批发酵工艺的优化[J].食品与发酵工业,2016,42(5):187-191.[27]张颖,沈业寿.液体发酵蜜环菌培养条件的优化及菌体多糖的分离[J].食用菌,2001(2):5-7.[28]程显好,刘林德,董洪新,等.蜜环菌菌丝体液体培养条件的优化[J].中药材,2007,30(5):509-512.[29]贺建超,李利军,卢美欢,等.液体培养富硒蜜环菌的研究[J].陕西农业科学,2018,64(4):33-35.[30]唐兴国,王伯诚,赖小芳.天麻共生蜜环菌液体种培养基的优化[J].浙江农业科学,2017,58(4):635-637.

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Last Update: 2021-01-13