|Table of Contents|

Establishment of Regeneration System of ‘Guidong No.1’ Asparagus cochinchinensis

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2020年20
Page:
112-118
Research Field:
Publishing date:

Info

Title:
Establishment of Regeneration System of ‘Guidong No.1’ Asparagus cochinchinensis
Author(s):
YU Weihua; SHI Qian; ZHANG Xiangjun; PAN Yingnan; MENG Ping; LI Ting
(Institude of Biotechnology,Guangxi Academy of Agricultural Sciences,Nanning,Guangxi 530007)
Keywords:
‘Guidong No.1’ Asparagus cochinchinensis; improved varieties; tissue culture; regeneration system
PACS:
-
DOI:
10.11937/bfyy.20194919
Abstract:
The stem segments of Asparagus cochinchinensis were used as materials.The effects of different proportions of auxin NAA and cytokinin 6-BA on the dedifferentiation,re-differentiation,bud proliferation and adventitia root induction in the bud organs of Asparagus cochinchinensis were studied.This study provided a reference for industrial breeding and effective protection of wild germplasm resources of Asparagus cochinchinensis.The results showed that the best sterilization effect of stem segments was 10 minutes with 0.1% HgCl2,and the rate of aseptic living was 76.08%.The optimal primary culture medium was MS+6-BA 1.0 mg?L-1+NAA 0.10-0.20 mg?L-1,explants completed the process of redifferentiation and de-differentiation in one culture cycle (30 days),and the bud induction rate was above 83.00%.The bud proliferation coefficients were the highest(3.41) in MS medium supplemented with 6-BA 0.5 mg?L-1 and IAA 0.1 mg?L-1.The highest root formation frequency (85.55%),average number of roots (5.0),and root length (2.2-3.1 cm) were achieved on 1/2MS medium containing NAA 1.50 mg?L-1 and IBA 1.0 mg?L-1.

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Last Update: 2021-01-08