|Table of Contents|

Identification of Codonopsis pilosula Powdery Mildew New Fungi andGermination Characteristics of Conidia

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2020年15
Page:
111-117
Research Field:
Publishing date:

Info

Title:
Identification of Codonopsis pilosula Powdery Mildew New Fungi andGermination Characteristics of Conidia
Author(s):
XU Xuefen; NI Chunhui; LI Huixia; LI Jinhong; LI Wenhao; XU Zhipeng
(College of Plant Protection,Gansu Agricultural University/Biocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province,Lanzhou,Gansu 730070)
Keywords:
powdery mildew on Codonopsis pilosula; rDNA-ITS sequence; 28S D1/D2 gene; P.erigerntis-canadensis; conidia germination
PACS:
-
DOI:
10.11937/bfyy.20194043
Abstract:
Taking the powdery mildew leaves of Codonopsis pilosula collected in Longxi County of Gansu Province as test materials, the pathogen species were identified by morphological observation, rDNA-ITS sequence and 28S D1/D2 gene sequencing, and spore germination test was conducted by suspension drop method. In order to clarified the pathogen species and conidia germination characteristics of Codonopsis pilosula in this area, and provided reference for disease control. The results showed that the mycelium of the pathogen was 2－8 μm thick and had papillae appressorium; the ascocarps were approximately spherical with 5－17 accessory filaments, most of which were on one side of the ascocarp and were 23－131 μm long, 0.19－1.51 times of diameter of the ascocarps; it contained one ascus with eye diameters of 4－15 μm and 8 ascospores per ascus. The optimum temperature for conidia germination was 25 ℃, and the optimum pH was 6. The tested four carbon sources had no effect on conidia germination. Continuous light (more than 40 hours) had great inhibition on conidia germination. All the morphological characteristics were identified with Podosphaera erigerntis-canadensis. The rDNA-ITS sequence and 28S D1/D2 gene were clustered with P.erigerntis-canadensis by MEGA 6.0 software analysis. The similarities with KJ660809 and AB462764 were 99.62% and 99.82% respectively. The powdery mildew of Codonopsis pilosula caused by P.erigerntis-canadensis had not been reported in China.

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Last Update: 2020-10-29