|Table of Contents|

Screening of Strawberry Mutants Resistant to Anthracnose Disease Using Ethyl Methane Sulphonate (EMS) and Toxin Mutagenesis Techniques

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2020年01
Page:
7-12
Research Field:
Publishing date:

Info

Title:
Screening of Strawberry Mutants Resistant to Anthracnose Disease Using Ethyl Methane Sulphonate (EMS) and Toxin Mutagenesis Techniques
Author(s):
ZHANG Qinghua; GUO Cong; XIANG Fayun; ZENG Xiangguo; LIU Qiaolin; HAN Yongchao
(Institute of Industrial Crops,Hubei Academy of Agricultural Sciences,Wuhan,Hubei 430064)
Keywords:
straberry anthracnose; ethyl methane sulphonate (EMS); crude extracts; resistant mutants
PACS:
-
DOI:
10.11937/bfyy.20191871
Abstract:
The petioles of strawberry cultivar ‘Jingyao’ in vitro were treated with chemical mutagen ethyl methane sulfonate（EMS）and the crude toxin produced by Colletotrichum siamense Nj-2，the pathogenic strain of straberry anthracnose.As a result，the system of in vitro screening some resistant mutants for strawberry anthracnose was established and the resistant mutants were obtained.The results showed that the petioles were treated with 0.8% of EMS for 2 hours which resulted in the effect of the ‘semi lethal dose’,and the appropriate selection pressure was that the petioles were cultured in medium supplemented with 4% of Nj-2 crude extracts.There were 43 regenerated buds survival in 532 mutation and after twice discontinuous screening the survival rate was 8.1%,and of which 12 strains were identified as resistant mutants after inoculated with C.siamense isolate Nj-2 on leveas and petioles of mutans in vitro.This study provided materials for further study on the mechanism of strawberry anthracnose resistance.

References:

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Last Update: 2020-01-13