|Table of Contents|

Study on Cymbidium tracyanum var. huanghua×Cymbidium mastersii on Virus Elimination Using Vitrification-cryopreservation Technology

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2020年05
Page:
61-66
Research Field:
Publishing date:

Info

Title:
Study on Cymbidium tracyanum var. huanghua×Cymbidium mastersii on Virus Elimination Using Vitrification-cryopreservation Technology
Author(s):
WANG Yuying1; WANG Qin1; LI Zhilin1; WEI Quantao1; SHANG Zhengrui1; LING Qing2
(1.College of Garden and Horticulture,Yunnan Agricultural University,Kunming,Yunnan 650201；2.Yunnan Agricultural Vocational and Technical College,Kunming,Yunnan 650031)
Keywords:
Cymbidium tracyanum var.huanghua×Cymbidium mastersii; protocorm; vitrification; cryopreservation; virus elimination
PACS:
-
DOI:
10.11937/bfyy.20191600
Abstract:
The original bulbs of the Cymbidium tracyanum var.huanghua×Cymbidium mastersii tissue culture were used as the test material.The vitrification ultra-low temperature method was used to study the removal of Cymbidium virus.It would provide reference for virus elimination technology of orchids.This experiment was a study about virus-elimicaton of orchids using vitrification-cryopreservation technology,and the tissue culture protocorm of Cymbidium tracyanum var.huanghua×Cymbidium mastersii was used as the experiment material.The results showed that pre-culture for 4 days in 0.5 mol·L-1sucrose concentration,then treat 50 minutes in loading liquid that with sucrose concentration of 0.6 mol·L-1 on ice,the next step was shift into PVS-2 liquid and vitrification treatment 120 minutes on ice,then freeze 40 minutes in liquid nitrogen,after that thaw 3 minutes in 37 ℃ centigrade for 3 minutes,finally load-off 20 minutes in unloading liquid (1/2MS+1.2 mol·L-1 sucrose).After resuming culture,the survive rate of protocorm could reach more than 65%,and the detoxification rate could reach 97% by random detection of different samples.

References:

[1]罗毅波,贾建生,王春玲.中国兰科植物保育的现状和展望[J].生物多样性,2003(1):70-77.[2]陈心启.兰花与昆虫传粉[J].植物杂志,1988(6):30-32.[3]吴应祥.中国兰花资源及其保护[J].中国花卉盆景,1987(2):6-7.[4]牛田,张林,王厚新,等.国兰育种研究进展[J].山东农业科学,2013,45(7):129-132.[5]石镇源,唐敏,杨红飞,等.LED不同光质对虎雪兰组培苗生理生化特性影响的研究[J].云南农业大学学报(自然科学版),2012,27(6):863-869.[6]王华,赵凤,杨丽华,等.3种叶面肥对虎雪兰光合生理特性的影响[J].西南林业大学学报,2016,36(1):149-152.[7]王婷婷.兰花病毒病的诊断与防治[J].黑龙江科技信息,2013(19):279.[8]沈淑琳.兰花病毒的种类、检验和防治[J].植物检疫,1988(2):144-150.[9]LEE C H,ZHENG Y X,JAN E J.The orchid-infecting viruses found n the 21st century[M]//Chen W H,Chen H H.Orchid Biotechnology III.Singapore:World Scientific Publishin,2015:145-157.[10]张建军,谢为龙.兰花病毒病研究进展[J].植物检疫,1999(2):47-49.[11]明艳林,郑金龙,郑国华,等.兰花抗病毒基因工程研究进展(综述)[J].亚热带植物科学,2010,39(1):92-96.[12]陈芳,王子成.植物脱毒新技术:低温疗法[J].河南农业科学,2006(12):11-13,81.[13]黄修梅,惠霖,袁春爱,等.超低温疗法脱除马铃薯种薯病毒病的研究进展[J].种子,2015,34(3):50-54.[14]BRISON M,DE BOUCAUD M T,PIERRONNET A.Effect of cryopreservation on the sanitary state of a cv Prunus rootstock experimentally contaminated with plum pox potyvirus[J].Plant Science,1997,123(2):189-196.[15]盛宏亚,万继花,徐川,等.利用玻璃化超低温技术脱除草莓斑驳病毒(SMoV)的初步研究[J].中国农业大学学报,2016,21(3):53-57.[16]蔡斌华,张计育,渠慎春,等.通过玻璃化超低温处理脱除草莓轻型黄边病毒(SMYEV)研究[J].果树学报,2008(6):872-876.[17]冯超红.苹果茎尖超低温保存技术及脱毒效率的研究[D].杨凌:西北农林科技大学,2014.[18]靳慧洁.东方百合病毒检测与茎尖超低温脱毒研究[D].福州:福建农林大学,2009.[19]李涵,陆琳,翟素萍,等.杂交兰种苗超低温脱毒技术研究[J].中国农业科技报道,2018,20(1):147-153.[20]贺嘉.蝴蝶兰(Phalaenopsis)脱毒快繁关键技术及其生理基础的研究[D].南京:南京农业大学,2010.[21]邱静,汤浩茹,曹会娟,等.园艺植物茎尖冷冻疗法脱毒的技术研究[J].植物生理学报,2014,50(1):1-6.[22]范建芝,井水华,杨淑娟,等.低温疗法脱除植物病毒研究进展[J].山东农业科学,2012,44(11):106-111.[23]曹桦,李涵,李绅崇,等.一种石斛超低温保存脱毒的方法[P].CN105706922A,2016-06-29.[24]ELLIOTT G D,WANG S P,FULLER B J.Cryoprotectants:A review of the actions and applications of cryoprotective solutes that modulate cell recovery from ultra-low temperatures[J].Cryobiology,2017,76:74-91.[25]沈阳,吕文刚,洪旭,等.侵染兰花的病毒种类及其检疫重要性[J].植物检疫,2019,33(1):1-7.[26]林志楷,郭莺,刘黎卿.兰花病毒检测技术研究进展(综述)[J].亚热带植物科学,2010,39(3):87-92.[27]施农农,徐莺,王慧中,等.复合感染建兰花叶病毒和齿兰环斑病毒的兰花超微结构观察及病原物快速鉴定[J].分子细胞生物学报,2007(2):153-163.[28]扆守鑫,谭冠林,李凡,等.云南部分兰花病毒病的病害调查及病原鉴定[J].云南农业大学学报,2008(3):325-328.

Memo

Memo:
-
Last Update: 2020-04-23