|Table of Contents|

Optimization of Extraction and Characterization of Polysaccharide From Stropharia rugosoannulata Fermentation Mycelium

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2020年01
Page:
111-116
Research Field:
Publishing date:

Info

Title:
Optimization of Extraction and Characterization of Polysaccharide From Stropharia rugosoannulata Fermentation Mycelium
Author(s):
WANG MixueSHENG ZhicunCHEN Xiaolan
(Jiangsu Agri-animal Husbandry Vocational College,Taizhou,Jiangsu 225300)
Keywords:
Stropharia rugosoannulatamycelium polysaccharideextraction processstructural characterization
PACS:
-
DOI:
10.11937/bfyy.20191112
Abstract:
In order to study the optimum extraction conditions and preliminarily characterization of polysaccharide from fermentation mycelium of Stropharia rugosoannulata.Box-Behnken design of response surface method was employed to optimize the extraction process.The results showed that the liquid-solid ratio,extraction temperature,extraction time of optimum process conditions were 26∶1 mL?g-1,82 ℃,3.5 hours,respectively.Under these conditions,the polysaccharides yield was 15.12%.The monosaccharide composition analysis by pre-column derivatization HPLC method showed that the mycelium polysaccharide was consisted of mannose,ribose,rhamnose,glucose,xylose,arabinose and fructose in a molar ratio of 0.76∶1.64∶0.65∶1.00∶1.24∶1.18∶0.20.Meanwhile,infraredspectra showed the polysaccharide had a typical characteristic absorption of sugars.The results of this study would provide a theoretical basis for the development and utilization of mycelium from liquid fermentation of Stropharia rugosoannulata.

References:

[1]黄年来.大球盖菇的分类地位和特征特性[J].食用菌,1995,17(6):11.[2]萨仁图雅,图力古尔.大球盖菇研究进展[J].食用菌学报,2005,12(4):57-64.[3]佘冬芳,樊卫国,徐彦军.白灵菇和大球盖菇的营养分析与比较[J].食用菌,2007,29(4):57-58.[4]王晓炜,詹巍,陶明煊,等.大球盖菇营养成分、抗氧化活性物质分析[J].食用菌,2007,29(6):62-63.[5]陈君琛,李治彬,吴俐,等.大球盖菇黄酮类化合物提取及抑菌性研究[J].北京工商大学学报(自然科学版),2010,28(6):9-13.[6]ZHAI X H,ZHAO A J,GENG L J,et al.Fermentation characteristics and hypoglycemic activity of an exopolysaccharide produced by submerged culture of Stropharia rugosoannulata[J].Annals of Microbiology,2013,63(3):1013-1020.[7]ZHANG W,TIAN G,GENG X,et al.Isolation and characterization of anovellectin from the edible mushroom Strophariarugosoannulata[J].Molecules,2014(19):19880-19891.[8]闫静,周祖法,宋吉玲.竹林套种大球盖菇优质高效栽培技术[J].2017,36(3):76-77,80.[9]暴增海,邱传庆,王增池,等.大球盖菇部分生物学特性研究[J].北方园艺,2009(1):217-219.[10]陈君琛,赖谱富,周学划,等.响应面法优化大球盖菇粗多糖提取工艺[J].食品科学,2012,33(2):139-142.[11]陈婵,黄靖,丁玲.超声波辅助提取大球盖菇总黄酮工艺优化[J].食品研究与开发,2015,36(24):116-119.[12]庞振凌,田龙.大球盖菇多糖发酵工艺优化研究[J].江苏农业科学,2007(1):157-158.[13]曾健勇,赵博,毕文玉,等.Na2SeO3和ZnSO4对大球盖菇富集液体培养的影响[J].吉林农业大学学报,2018,40(2):171-177.[14]DUBOI M,GILLES K A,HAMILTON J K,et al.Colorimetric method for determination of sugars and related substances[J].Analytical Chemistry,1956,28(3):350-356.[15]HAO L M,SHENG Z C,LU J K,et al.Characterization and antioxidant activities of extracellular and intracellular polysaccharides from Fomitopsis pinicola[J].Carbohydrate Polymers,2016,141:54-59.[16]钟丽霞,江震宇,汪嘉妮,等.山楂多糖提取工艺优化及其降血糖、降血脂活性研究[J].食品工业科技,2019,40(13):119-124,147.[17]陈婵,黄靖,丁玲.超声波辅助提取大球盖菇总黄酮工艺优化[J].食品研究与开发,2015,36(24):116-119.[18]李松林,王林,叶华,等.栉江珧粗多糖提取工艺优化、组成分析及其抗氧化活性研究[J].食品工业科技,2018,39(4):177-182,209.[19]陈君琛,翁敏劼,赖谱富,等.大球盖菇多糖的分子质量分布及其单糖的组成[J].中国农业科学,2011,44(10):2109-2117.[20]顾亚萍,钱和.茶叶多糖纯度鉴定及结构分析的研究进展[J].食品研究与开发,2008,28(7):166-170.[21]夏朝红,戴奇,房韦,等.几种多糖的红外光谱研究[J].武汉理工大学学报,2007,29(1):45-47.[22]孙延芳,李子昂,梁宗锁,等.食用菌多糖及其红外光谱分析[J].黑龙江农业科学,2011(10):99-100.

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Last Update: 2020-01-14