|Table of Contents|

Establishment of Agrobacterium-mediated Genetic Transformation System in Lycium ruthenicium Murr.

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2020年01
Page:
104-110
Research Field:
Publishing date:

Info

Title:
Establishment of Agrobacterium-mediated Genetic Transformation System in Lycium ruthenicium Murr.
Author(s):
WANG Jing; TANG Jing; CUI Yueting; CHEN Yun; ZHAO Huijun; YAN Xingfu
(Key Laboratory of Ecosystem Model and Applications of State Nationalities Affairs Commission/College of Biological Science and Engineering,North Minzu University,Yinchuan,Ningxia 750021)
Keywords:
Agrobacterium; Lycium ruthenicium Murr.; hypocotyl; genetic transformation
PACS:
-
DOI:
10.11937/bfyy.20191730
Abstract:
Using Lycium ruthenicium Murr.hypcotyls as material,a method of Agorobacterium-mediated genetic transformation system for further analyzing gene function was established，in order to provide a reference for further study on the function of Lycium ruthenicium gene.The results showed that the vector pORE R1-[STBX]35S:GUS:NOS[STBZ] was constructed and transformed into Agorobacterium GV3101.Hypocotyls of Lycium ruthencum Murr.were infected with Agrobacterium suspension,OD600=0.5.After selective cultivation,PCR and GUS activity detection,identified the positive plants,with transformation rate was 16%.Transformed plants were all well growing.The results indicated that Agrobacterium could successful transform the genes into hypocotyls of Lycium ruthenicium Murr.,with highly transformation rate.

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Last Update: 2020-01-14