|Table of Contents|

Chromosome Preparation Optimization and Karyotype Analysis of Two Landscape Plants in Araliaceae

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2019年22
Page:
83-89
Research Field:
Publishing date:

Info

Title:
Chromosome Preparation Optimization and Karyotype Analysis of Two Landscape Plants in Araliaceae
Author(s):
ZHAO Yan; DU Kanghua; LI Wanxuan; SHAO Yandong; ZHANG Jie
(Collage of Landscape and Horticulture,Yunnan Agricultural University,Kunming,Yunnan 650201)
Keywords:
Metapanax dalavayi; Fatsia japonica; chromosome; preparation optimization; karyotype analysis
PACS:
-
DOI:
10.11937/bfyy.20184511
Abstract:
The unexpanded leaf tips of Metapanax dalavayi and Fatsia japonica,two landscape plants in Araliaceae,were used as materials in this study.The chromosome staining factors were optimized and karyotype characteristics were also investigated.The results showed that the best pretreatment solution of Metapanax dalavayiis 0.002 mol?L-1 8-HQ+0.04% actidione,whereas 0.001 mol?L-1 8-HQ was the best for Fatsia japonica.The rest optimization conditions of this two testing plants were sample at 09:00—09:30 a.m.,fixing in Carnoy′s solution (one part glacal acetic acid and three parts 95% ethanol) for 12-24 hours.The fixed material was macerated in dissociation liquid (the volume ratio of anhydrous alcohol and hydrochloric acid was 1 to 1) for 13 minutes,and then stained for 10 minutes with carbolfuchsin solution.The karyotype of Metapanax dalavayi and Fatsia japonicawere 2B (2n=48=28m+18sm+2st) and 2A (2n=48=34m+14sm),and the karyotype asymmetry index (As.K) were 61% and 60%,respectively.Compared the results with those from the reported plants in Araliaceae,these two testing plants were relatively primitive species.These results would enrich the awareness of genetic composition of Metapanax dalavayi and Fatsia japonica,and provide cytological evidence for the breeding of these two testing plants.

References:

[1]WEN J,PLUNKETT G M,MITCHELL A D,et al.The evolution of Araliaceae:A phylogenetic analysis based on ITS sequences of nuclear ribosomal DNA[J].Syst Bot,2001,26:144-167.[2]WEN J,ZHU Y P,LEE C,et al.Evolutionary relationships of Araliaceae in the Malesian region:A preliminary analysis[J].Acta Bot Yunnanica,2008,30:391-399.[3]PLUNKETT G M,WEN J,LOWRY P P.Infrafamilial classifications and characters in Araliaceae:Insights from the phylogenetic analysis of nuclear(ITS) and plastid (trnL-trnF) sequence data[J].Plant Systematics & Evolution,2004,245(1-2):1-39.[4]LI R,MA P F,WEN J,et al.Complete sequencing of five Araliaceae chloroplast genomes and the phylogenetic implications[J].PLoS One,2013,8(10):e78568.[5]向其柏,季春峰.五加科大参属的两个新组合:评梁王茶属“Nothopanax”或Metapanax之归属问题[J].南京林业大学学报(自然科学版),2006,30(6):41-43.[6]中国科学院中国植物志编委会.中国植物志.第54卷[M].北京:科学出版社,1978.[7]葛菲,宋子荣.梁王茶的镇痛作用研究[J].中国野生植物资源,2000(3):50-51.[8]杨青,张健,欧阳胜,等.掌叶梁王茶茎皮的化学成分研究[J].中国中药杂志,2014,39(10):1858-1862.[9]洪化鹏,程光中.梁王茶成份研究Ⅰ梁王精油组份初探[J].贵州师范大学学报(自然科学版),1991(2):28-31.[10]解天龙,赵嘉德,业荣.澄江县野生蔬菜梁王茶的开发利用[J].农村实用技术,2015(11):14-15.[11]刘扬.城市道路八角金盘叶片重金属Pb、Zn污染研究:以上海漕溪路-沪闵路、延安西路为例[D].上海:上海师范大学,2007.[12]梁志远,甘秀海,王道平,等.八角金盘茎、叶、花(果实)中精油的化学成分分析[J].安徽农业科学,2012,40(15):8473-8475.[13]王俊,马玉心,崔大练,等.八角金盘传粉昆虫及其访花行为[J].应用昆虫学报,2011(3):764-768.[14]丁雪娇.八角金盘(Fatsia japonica(Thunb.) Decne.et Planch.)传粉生物学及胚胎学研究[D].舟山:浙江海洋学院,2014.[15]CHIANG T,CHEN S,KATO H,et al.Temperate origin and diversification via southward colonization in Fatsia(Araliaceae),an insular endemic genus of the West Pacific Rim[J].Tree Genetics & Genomes,2014,10(5):1317-1330.[16]张力鹏,刘博,陈成彬.植物染色体分子核型技术研究进展[J].北方园艺,2014(8):179-183.[17]王梦叶,杜晓华,贾文庆,等.角堇与大花三色堇染色体核型分析[J].西北植物学报,2018,38(2):258-263.[18]李磊,沈吉焚,何梦玲,等.小花吊兰染色体制片优化与核型分析[J].北方园艺,2016(1):76-79.[19]张力鹏,郏书行,徐仲凯,等.六个金银花栽培品种的核型分析[J].北方园艺,2017(6):103-107.[20]OEHM G.Studien über riesen-und zwergformen einheimischer pflanzen[J].Beih Bot Zentralbl,1924,40:237-294.[21]YI T,PLUNKETT G M,LOWRY P P,et al.Chromosomal evolution in araliaceae and close relatives[J].Taxon,2004,53(4):987-1005.[22]HSU P S.Index to plant chromosome numbers reported in Chinese literature[J].III Invest Stud Nat,1990,9:81-87.[23]HSU P S.Index to plant chromosome numbers reported in Chinese literature[J].Invest Stud Nat,1985,5:111-116.[24]SHARMA A K,SHARMA A.Chromosome techniques:Theory and practice[M].3ed. London:Butterworths,1980.[25]李懋学,陈瑞阳.关于植物核型分析的标准化问题[J].武汉植物学研究,1985(4):297-302.[26]LEVEN A,FRED A K,SANDBERG A A.Nomenclature for centromeric position on chromosomes[J].Heredities,1964,52:201-220.[27]KUO S R,WANG T T,HUANG T C.Karyotype analysis of some Formosan gymnosperms[J].Taiwania,1972,17(1):66-80.[28]ARANO H.Cytological studies in subfamily Carduoideae(Compositae) of Japan I X.The karyotype analysis and phylogenetic considera-tions on Pertya and Ainsliaea[J].Botanic Magazine(Tokyo),1963,76:32-40.[29]STEBBINS G L.Chromosomal evolution in higher plants[M].London:Edward Arnold,1971:87-89.[30]李懋学.植物染色体研究技术[M].哈尔滨:东北林业大学出版社,1991.[31]刘永安,冯海生,陈志国,等.植物染色体核型分析常用方法概述[J].贵州农业科学,2006,34(1):98-102.[32]李庆玲,陈荣,杨跃生,等.两种天南星科植物根尖组织染色体观察方法的优化[J].北方园艺,2012(2):134-136.[33]夏雪,辜金花,刘丹,等.黄花芥蓝染色体制片优化及核型分析[J].浙江大学学报(农业与生命科学版),2016,42(5):527-534.[34]唐艳梅,易自力,邓果特,等.4种芒属植物的核型分析及亲缘关系探讨[J].湖南农业大学学报(自然科学版),2018,44(5):487-494.[35]任艳,王辉,石延茂,等.不同预处理对花生根尖细胞有丝分裂制片的影响[J].花生学报,2008,37(2):28-31.[36]胡伸萌,孙文光,李志敏.预处理液对制片优化及染色体形态的影响：以雅砻江冬麻豆为例[J].云南师范大学学报(自然科学版),2018(1):51-57.[37]高和琼,王英,金鸽,等.橡胶树叶片染色体制片方法的优化[J].热带作物学报,2009,30(5):565-569.[38]王明艳,韩秀玲,吴鹏,等.药用植物刺五加染色体核型分析[J].生物学通报,2011,46(4):48-49.[39]梁韶,宋娟,雷秀娟,等.四种人参属药用植物的核型分析[J].人参研究,2017,29(2):6-10.[40]崔秋华.人参染色体核型及Giemsa染色的研究[J].吉林农业大学学报,1984(1):3-10.[41]梁韶.三种人参属药用植物荧光原位杂交技术及核型分析[D].北京:中国农业科学院,2017.[42]SUN B Y,KIM C H,SOH W Y.Chromosome numbers of Araliaceae in Korea[J].Korean Journal of Plant Taxonomy,1988,18:291-296.[43]KITAMURA S,MURATA G.Colored Illustrations of woody plants of Japan[M].Japan:Hoikusha Publish Co.Ltd.,1982.[44]陈琴怡.两种五加科植物的叶绿体全基因组研究及其系统发育分析[D].杭州:浙江大学,2017.

Memo

Memo:
-
Last Update: 2019-12-11