|Table of Contents|

Optimization of Factors Affecting Induction and Differentiation of Bud Segments of Eucommia ulmoides Oliver

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2019年05
Page:
32-38
Research Field:
Publishing date:

Info

Title:
Optimization of Factors Affecting Induction and Differentiation of Bud Segments of Eucommia ulmoides Oliver
Author(s):
LI Xiang1; GUO Jinping2; ZHANG Yunxiang1
(1.College of Forestry，Shanxi Agricultural University，Taigu，Shanxi 030801;2.College of Graduate Studies，Shanxi Agricultural University，Taigu，Shanxi 030801)
Keywords:
Eucommia ulmoides Oliver; tissue culture; stem segment; axillary bud induction
PACS:
-
DOI:
10.11937/bfyy.20182802
Abstract:
The young stem segments of Eucommia ulmoides Oliver were used as materials，tissue culture method was used，the effects of disinfection time，basic medium，NAA concentration，6-BA concentration，low temperature treatment time and culture environment on the induction of Eucommia ulmoides Oliver axillary buds and the differentiation of Eucommia ulmoides Oliver were investigated，in order to provide a basis for establishing an efficient tissue culture system of Eucommia ulmoides Oliver.The results showed that the best disinfection time was 9 minutes，and the rate of pollution and mortality were lower，10.00% and 26.67% respectively;the basic medium for axillary buds induction was MS medium，with an induction rate of 91.66%;when the induction medium was MS+0.2 mg?L-1 6-BA+0.03 mg?L-1 NAA，the induction rate of axillary buds was 93.33% and it grew well;when the low temperature treatment time was 24 hours，explant induction effect was the best，and browning rate was the lowest，5.00%;compared with dark culture，light culture conditions were more conducive to the induction and growth of axillary buds of Eucommia ulmoides;MS+1.5 mg?L-1 6-BA+0.1 mg?L-1 NAA was the best medium for callus differentiation，MS+2.5 mg?L-1 6-BA+0.1 mg?L-1 NAA was the best medium for bud proliferation.

References:

[1]张志翔.树木学(北方本)[M].北京:中国林业出版社,2008:152-153.[2]康向阳.杜仲良种选育研究现状及展望[J].北京林业大学学报,2017,39(3):1-6.[3]童孝茂.杜仲的栽培技术和开发利用[J].安徽农学通报,2007(20):117-118.[4]朱翠英,张真,谢经霞,等.杜仲组培快繁技术的研究[J].宁夏农林科技,2005(6):32-33.[5]张康健.中国杜仲研究[M].西安:陕西科技出版社,1992:21[6]LI Z Y,GU J,YUN J,et al.Hypertensive cardiac remodeling effects of lignan extracts from Eucommia ulmoides Oliv.bark:A famous traditional Chinese medicine[J].The American Journal of Chinese Medicine,2003,41(4):801-815.[7]李琰,张靖,辛转霞,等.杜仲组培快繁的研究[J].西北农林科技大学学报(自然科学版)，2004,23(6):79-82.[8]罗丽,赵德刚.杜仲带芽茎段的组织培养[J].江苏农业科学,2007(5):174-176.[9]唐亮,金晓玲.杜仲组织培养的研究进展[J].贵州农业科学,2010，38(3):15-18.[10]孟长军.组培实验室常见消毒方法优劣比较[J].中国园艺文摘,2012，28(8):177-178，168.[11]黄作喜,邱超,曾桢迦,等.植物组织培养中消毒剂的应用研究进展[J].内江师范学院学报,2013，28(6):26-30.[12]李素华,何松林.基本培养基和激素对常青白蜡腋芽诱导的影响[J].贵州农业科学,2009，37(12):175-176.[13]郑永春,迟丽华.笃斯越橘组培苗增殖扩繁培养基配方筛选试验[J].北方园艺,2013(9):138-139.[14]孙清荣,王金政,薛晓敏,等.‘金凯特’杏的组织培养与快繁[J].山东农业科学,2016，48(12):25-28.[15]德永军,吕涛,王一,等.文冠果茎段形成层不定芽诱导组织培养技术初探[J].内蒙古农业大学学报(自然科学版),2014,35(2):39-42.[16]张桂琴,徐祥龄,赵志学.文冠果嫩茎组织诱导植株移栽初获成功[J].林业科技通讯，1980(7):4-5.[17]张娜,张芸香,郭晋平.文冠果腋芽诱导关键影响因素与培养体系优化[J].山西农业大学学报(自然科学版),2014,34(1):53-58.[18]李琰,张存莉,严忠海,等.不同培养基对杜仲愈伤组织诱导和生长的影响[J].西北林学院学报,2003(3):37-39.[19]高国训.植物组织培养中的褐变问题[J].植物生理学通讯,1999(6):501-506.[20]黄烈健,王鸿.林木植物组织培养及存在问题的研究进展[J].林业科学研究，2016，29(3):464-471.[21]邢阿宝,崔海峰,俞晓平,等.光质及光周期对植物生长发育的影响[J].北方园艺,2018(3):163-172.[22]SKOOG F，MILLER C O.Chemical regulation of growth and organ formation in plant tissues cultured in vitro[J].Symp Soc Exp Biol，1957,54:118-130.[23]谷文英,裴德清,朱登云,等.杜仲茎段培养的初步研究[J].莱阳农学院学报,1999(1):6-9.

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Last Update: 2019-03-28