|Table of Contents|

Cloning and Expression of CHR Gene in Astragalus membranaceus

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2019年06
Page:
141-146
Research Field:
Publishing date:

Info

Title:
Cloning and Expression of CHR Gene in Astragalus membranaceus
Author(s):
BAO Rui; LIU Junpin; YUAN Yuan; YU Yang; QUAN Xueli; WU Songquan
(Agricultural College,Yanbian Uniersity，Yanji，Jilin 133000)
Keywords:
Astragalus membranaceus (Fisch.) Bge.; chalcone reductase; gene cloning; expression analysis
PACS:
-
DOI:
10.11937/bfyy.20182579
Abstract:
A CHR gene was cloned from Astragalus membranaceus in the present study，the GenBank accession number was KY086286 (AmCHR)，the full-length cDNA of AmCHR was 1 173 bp and it consisted of 957 bp open reading frame encoding 318 amino acids，which predicated molecular weight was 35.58 KDa and isoelectric point was 6.26.Bioinformatics analysis showed that AmCHR was homology with other plant CHR.Real-time PCR and HPLC indicated that the expression levels of AmCHR was similar to the changes of CG contents in different tissues.These results showed that the cloned AmCHR was a new CHR gene and the expression of AmCHR might be relevant to CG accumulation in A.membranaceus.

References:

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Last Update: 2019-04-22