Characteristic Comparison Among Diploid,Tetraploid and Octaploid Plants and Obtaining of Pataploid and Aneuploid in Echinacea purpurea L.(PDF)
《北方园艺》[ISSN:1001-0009/CN:23-1247/S]
- Issue:
- 2019年02
- Page:
- 138-145
- Research Field:
- Publishing date:
Info
- Title:
- Characteristic Comparison Among Diploid,Tetraploid and Octaploid Plants and Obtaining of Pataploid and Aneuploid in Echinacea purpurea L.
- Author(s):
- HUANG Huaxi1; CHEN Rong1; 2; YANG Yuesheng3; WU Hong3
- (1.College of Biology and Pharmacy,Yulin Normal University,Yulin,Guangxi 537000;2.College of Chinese Medicinal Materials,Jilin Agricultural University,Changchun,Jilin 130118;3.Guangdong Technology Research Center for Traditional Chinese Veterinary Medicine and Natural Medicine,South China Agricultural University,Guangzhou,Guangdong 510642)
- Keywords:
- Echinacea purpurea; tetraploid; octaploid; aneuploid
- PACS:
- -
- DOI:
- 10.11937/bfyy.20181636
- Abstract:
- Via cultivation of diploid,tetraploid and octaploid plants sharing the same genome of E.purpurea,investigating the influences of gene dosage on the vegetative and reproductive growth and on the content of the main functional compound,seeds collected from octaploid were germinated and the numbers of chromosomes in the root tip cells of the seedlings were counted.In order to provide more material choices for genetic basic theory research and breeding of abundant resources of E.purpurea.The results showed that with the increase of ploidy,plant height became shorter,leaf length to width ratio and ray flower petal length to width ratio decreased,the numbers of tiller per plant were all the largest in tetraploid.The numbers of capitulum and inflorescence among annual plants of tetraploid and octaploid were significantly lower than those of diploid plants,the numbers of capitulum and inflorescence among biennial plants were all the largest in tetraploid and were significantly higher than the diploid and octaploid.The yields of whole plant of tetraploid were 232.6% higher than ocaploid,the yields of whole plant of diploid were 187.1% higher than ocaploid.The contents of total polyphenols acid,chlorogenic acid,caffeoyl tartaric acid and cichoric acid in the above ground part and underground part of plants of tetraploid and octaploid were significantly higher than those of diploid plants,the contents of chlorogenic acid,chicoric acid in the aboveground part of octaploid was higher than in tetraploid.Two in vitro plantlets grew from seeds that collected from octaploid were assessed for ploidy state,one with 55 chromosomes and was considered as pentaploid and the other with 53 chromosomes and was certified as aneuploid.This study indicateed that octaploid was too high a ploidy level to exhibit the advantage of yield and quality for E.purpurea,but this ploidy level still had value when was used as parents for crossing.Tetraploid seemed a suitable ploidy level that cultivation of tetraploid may achieve high benefit than conventional cultivation of diploid.The pentaploid and aneuploid of E.purpurea were of value in the production and used as a bridge for genetic improvement,and useful supplement to the creation of euploid and aneuploid materials.
Last Update: 2019-01-24