Abstract

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Title:: Polysaccharides in the Fruit Body of Grifola frondosa Cultivated With JUNCAO

Author(s):: CAO Xiuming; CAI Yangxing; LIN Zhanxi; (National Engineering Research Center of JUNCAO Technology,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002)

Keywords:: JUNCAO; Grifola frondosa; polysaccharide; optimum extracting technology; isolation and purification

Abstract:: The fruit bodies of Grifola frondosa were separately cultivated with JUNCAO powder（Miscanthus floridulus，Dicranopteris dichotoma and Neyraudia reynaudiana）and sawdust.To optimize the extraction technology of polysaccharides and mensurate the polysaccharides content from Grifola frondosa,the solid-liquid ratio,temperature,extraction time and extraction times were tested according to the orthogonal experimental design L9（34）.Then the purifition of the polysaccharides was tested by DEAE-Sephadex A-25 column chromatography method and SepHadex G-200 gel chromatography method.The results showed that the optimal extracting conditions of polysaccharides on Grifola frondosa fruit body were 1∶40 g·mL-1 material to water，100 ℃，2 hours and extract 2 times.Two components were obtained under the JUNCAO cultivation including C-GLPl and C-GLP2；and also two components were gotten under the traditional sawdust cultivation inculding S-GLP1 and S-GLP2.Purity identification showed that C-GLPl and S-GLP1 were single symmetrical peak.