Abstract

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Title:: Effect of Growth Regulator on Bud Differentiation of Callus of Vaccinium uliginosum L.

Author(s):: LI Zheng; GAO Qingyu; ZHANG Bingxiu; ZHANG Yu; ZHANG Zhao; (College of Horticulture and Landerscape Architecture,Northeast Agricultural University,Harbin,Heilongjiang 150030)

Keywords:: Vaccinium uliginosum L.; callus; growth regulator; differentiation bud

Abstract:: Callus explants of the leaf and stem segments without buds of Vaccinium uliginosum L.SL1,which were compact,green and energetic,were used as the test materials in WPM medium.The effect of TDZ,ZT and 6BA,growth regulators of IBA,NAA and sucrose in different concentrations on bud differentiation were studied by using the singlefactor variable and multifactor orthogonal combination method in order to provide a reference for the preservation of germplasm resources and the selection of superior system in the future.The results showed that by using ZT alone to induce callus differentiation buds,ZT was more suitable for callus differentiation bud than TDZ and 6BA,and the best concentration was 2.0 mg·L-1,the callus differentiation rate of leaf and stem was up to 85.67% and 89.00%.When using TDZ,ZT,IBA and NAA,differentiation rate of leaf callus was better than that in the stem segment callus,and the rate of differentiation was up to 100.00% and 93.00%.The best components were TDZ 1.0 mg·L-1+ZT 3.0 mg·L-1+IBA 0.05 mg·L-1+NAA 0 mg·L-1.Sucrose concentration had effect on the bud differentiation of callus of leaf and stem segment,the best sucrose concentrations were 25 g·L-1 and 20 g·L-1.In conclusion,leaf callus was more suitable for differentiation bud formation,and the best medium was WPM+sucrose 20 g·L-1+agar 8 g·L-1+TDZ 1.0 mg·L-1+ZT 3.0 mg·L-1+IBA 0.05 mg·L-1.