Isolation and Identification of Wild Morels in Beijing(PDF)
《北方园艺》[ISSN:1001-0009/CN:23-1247/S]
- Issue:
- 2018年13
- Page:
- 145-152
- Research Field:
- Publishing date:
Info
- Title:
- Isolation and Identification of Wild Morels in Beijing
- Author(s):
- GUO Yuxin1; YIN Yuting1; JI Xinmeng1; CHEN Qingjun1; ZHANG Guoqing2; MA Xuan3
- (1.College of Plant Science and Technology，Beijing University of Agriculture/Beijing Key Laboratory for Agricultural Application and New Technique，Beijing 102206;2.College of Biological Sciences and Engineering，Beijing University of Agriculture，Beijing 102206;3.National Demonstration Center for Experimental Plant Production Education，Beijing University of Agriculture，Beijing 102206)
- Keywords:
- Beijing; wild Morchella; ITS sequence analysis; phylogeny
- PACS:
- -
- DOI:
- 10.11937/bfyy.20174507
- Abstract:
- The wild Morchella collected in Beijing was used as test material，by tissue separation method，CTAB method was used to extract Morchella DNA，agarose electrophoresis，ITS sequencing and molecular phylogenetic tree were conducted.Wild Morchella in Beijing mountainous area were isolated and identified，in order to provide basis for artificial cultivation of Morchella esculenta and utilization of microbial resources.The results showed that 7 isolates of Morchella were successfully isolated.The phylogenetic relationship of the isolates was very close.The mycelial morphology were similar，but the color depth，number of sclerotia，and mycelial growth rate were significantly different.Phylogenetic analysis showed that the wild morch collected in Beijing belonged to the yellow morel group.
Last Update: 2018-07-24