Abstract

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Title:: Spatial and Temporal Distribution of Apple Chlorotic Leaf Spot Virus in Apple Plant

Author(s):: LI Nan; WANG Yadi; YANG Junyu; DING Li; WANG Yanan; CAO Keqiang; (College of Plant Protection,Agricultural University of Hebei,Baoding，Hebei 071001)

Keywords:: apple chlorotic leaf spot virus; quantitative real-time RT-PCR; spatial and temporal distribution

Abstract:: The apple chlorotic leaf spot virus (ACLSV) samples collected from the apple orchard of Agricultural University of Hebei were used as materials,the real time fluorescence quantitative RT-PCR method was used to study the relationship between the different tissue type,the different direction of plant,the different stage of growth and development and the relative content of the virus,in order to provide references for defining the temporal and spatial distribution pattern of chlorotic leaf spot virus in apple plants.The results showed that from the view of tissue type,during full-blossom period,the relative concentration of ACLSV was the highest in flowers and the lowest in buds.The order from high to low was flowers,biennial branches bark,buds,annual branches bark and leaves.From the point of the direction of plant,using apple annual branches as material,the relative concentrations of ACLSV in four different directions were significantly different.The virus relative concentration in annual branch bark located in the south was the highest,the west was the lowest.From the view of the stage of growth and development,during the period of September 2015 to April 2016,using apple annual branches as material,the virus relative concentration increased from mid September to mid October.The relative concentration reached the peak in October and thereafter viral content began to decline and the trend showed upward in March.