|Table of Contents|

Microarray-based Analysis of Disease Resistance Signaling Hormones in Tomato(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2018年02
Page:
1-8
Research Field:
Publishing date:

Info

Title:
Microarray-based Analysis of Disease Resistance Signaling Hormones in Tomato
Author(s):
XU Ruirui
College of Biological and Agricultural Engineering,Weifang University/The 13th Five Year Key Laboratory of Biochemistry and Molecular Biology in Universities of Shandong,Weifang,Shandong 261061
Keywords:
tomatomicroarray analysishormonedisease resistanceexpression analysis
PACS:
-
DOI:
10.11937/bfyy.20173295
Abstract:
Tomato was used as test material,the Aglient chips and real-time fluorescent quantitative PCR technology were used to identificate differentially expressed genes (DEGs),especially disease resistance genes,after classic innate immunity-associated plant hormones ethylene(ET),methyl jasmonate (MeJA),and salicylic acid (SA) treatment,in order to further study on disease resistance signal hormone response,and provide some references for the synergy between signaling pathways.The results showed that 2 261,8 302,and 9 479 DEGs were identified in tomato after treatment with ET,MeJA,or SA,respectively.Gene ontology analysis indicated that these DEGs were associated with hormone and defense responses,developmental processes,signal transduction and protein metabolism pathways.Among these DEGs,178 up-regulated common DEGs (Co-DEGs) and 598 down-regulated Co-DEGs were identified.Aside from unknown Co-DEGs,481 known or predicted Co-DEGs were categorized on the basis of their functions.In addition,the expression of 10 DEGs was assessed via quantitative real-time polymerase chain reaction,and the results generally agreed with the Agilent microarray data.

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Last Update: 2018-01-26