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Separation and Identification of Trichoderma From the Rhizosphere Soil of Prunus padus

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2018年12
Page:
88-95
Research Field:
Publishing date:

Info

Title:
Separation and Identification of Trichoderma From the Rhizosphere Soil of Prunus padus
Author(s):
ZHOU ChangWANG JinjieFAN HaijuanYANG GuangqiLIU Zhihua
(College of Forestry,Northeast Forestry University,Harbin,Heilongjiang 150040)
Keywords:
Trichodermaisolationmorphological identificationdetermination of ITS sequencePrunus padus
PACS:
-
DOI:
10.11937/bfyy.20172427
Abstract:
The materials of the rhizosphere soil of bird cherry (Prunus padus L.) from the Academy of Agricultural Sciences Institute of Horticulture in Harbin and isolation methods of Rose Bengal medium and plate separation as well as identification methods of morphological classification presented by Gams&Bissett(1998) and molecular classification presented by Kullnig-Gradinger et al.(2002) were used to research the identity,number and variety of Trichoderma existed in the rhizosphere soil of bird cherry.The purpose of the research was to find the dominant species of Trichoderma in the rhizosphere soil of bird cherry.The results showed that 25 Trichoderma Harzianum strains isolated from the sample,which means there were large quality but only one species of Trichoderma spp.around the bird cherry root.The research results would provide Trichoderma resource for special Trichoderma biofertilizer of the bird cherry.

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Last Update: 2018-07-13