|Table of Contents|

Function of eIF-5A Gene in Apocynum venetum L．(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2017年23
Page:
190-193
Research Field:
Publishing date:

Info

Title:
Function of eIF-5A Gene in Apocynum venetum L．
Author(s):
WANG Lei1; 2; SUN Yao2; LI Yao2; WU Qiong2; XIA Xinli1
1.College of Biological Sciences and Technology,Beijing Forestry University,Beijing 100083;2.Institute of Advanced Technology,Heilongjiang Academy of Sciences,Harbin,Heilongjiang 150020
Keywords:
Apocynum venetum; eIF-5A; tobacco; stress resistance
PACS:
-
DOI:
10.11937/bfyy.20172087
Abstract:
To understand the effect of eIF-5A gene in response to salt stress,the eIF-5A cDNA fragment containing an ORF from Apocynum venetum was transformed into tobacco by Agrobacterium-mediated method.The POD activity,SOD activity and MDA content were measured between transgenic AveIF-5A tobaccos and wild type under normal condition and NaHCO3 stress.The results showed that there was no significant difference in POD activity and MDA content between transgenic and non-transgenic plants under normal condition but SOD activity of transgenic plants increased significantly.Under salt stress,transgenic tobacco over-expressing AveIF-5A gene showed an increase in POD and SOD activity,compared to wild lines.In contrast,transgenic tobacco displayed a decrease in MDA content under NaHCO3 stress.Overall,these results indicated that AveIF-5A gene played a critical role in salt tolerance in transgenic tobacco.

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Last Update: 2017-12-20