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Screening of Optimum Medium for Pollen Germination of Walnut(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2017年23
Page:
42-47
Research Field:
Publishing date:

Info

Title:
Screening of Optimum Medium for Pollen Germination of Walnut
Author(s):
ZHAO Shuang1; SU Yanping2; LI Han1; 3; ZHANG Xuemei1; 3; FAN Shuchang1; QI Guohui1; 3
1.College of Forestry,Agricultural University of Hebei,Baoding,Hebei 071000；2.College of Life Science，Langfang Teachers University,Langfang,Hebei 065000;3.Research Center for Walnut Engineering and Technology of Hebei,Lincheng,Hebei 054300
Keywords:
walnut; pollen; medium
PACS:
-
DOI:
10.11937/bfyy.20171760
Abstract:
Pollens of precocious walnut varieties ‘Lyuling’ and ‘Lyubo’ were used by culture method in vitro to study the effects of different proportion of sucrose,in order to determine the appropriate condition for walnut pollen germination and find out optimum medium,boric acid,calcium chloride on pollen germination.The results showed that the pollen germination rates of ‘Lyuling’ and ‘Lyubo’ on the medium with sucrose concentration of 160 g·L-1 were the highest,which were 57.23% and 48.09%,respectively.The optimum boric acid concentrations for pollen germination of ‘Lyuling’ and ‘Lyubo’ were 0.10 g·L-1 and 0.07 g?L-1;the optimum calcium chloride concentration for pollen germination of the two varieties was 0.20 g·L-1;the optimum medium for ‘Lyuling’ walnut pollen germination was as follows,160 g·L-1 sucrose,0.10 g·L-1 boric acid,0.20 g?L-1 calcium chloride, and the pollen germination rate of ‘Lyuling’ walnut on this medium reached 61.06%;the optimum medium for ‘Lyubo’ walnut pollen germination was as follows,160 g?L-1 sucrose,0.07 g?L-1 boric acid,0.20 g?L-1 calcium chloride,and the pollen germination rate of ‘Lyubo’ walnut on this medium reached 72.71%.

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Last Update: 2017-12-19