|Table of Contents|

Cloning and Expression Analysis of Adventitious Rooting Related  Gene PbARRO-1 of Pyrus betulifolia Bunge.(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2017年12
Page:
97-102
Research Field:
Publishing date:

Info

Title:
Cloning and Expression Analysis of Adventitious Rooting Related  Gene PbARRO-1 of Pyrus betulifolia Bunge.
Author(s):
WANG Wencheng; MA Chunhui; WANG Ran; GAO Yanan; LI Xiaoning; LI Dingli
(College of Horticulture，Qingdao Agricultural University/Qingdao Key Laboratory of Genetic Improment and Breeding in Horticultural Plants，Qingdao，Shandong 266109)
Keywords:
:Pyrus betulifolia ; adventitious rooting; PbARRO1; gene cloning; expression analysis
PACS:
-
DOI:
10.11937/bfyy.201712024
Abstract:
The leaves of Pyrus betulifolia were used as the materials，a novel adventitious rooting related oxygenase gene (ARRO gene) was cloned by Homology-based and RACE technology，and then analyzed with bioinformatics methods and quantitative real-time RT-PCR technology.The study laid a foundation for adventitious root formation studying.The results showed that the open reading frame of PbARRO-1 was 831 bp and encodes 276 amino acids，the molecular weight and isoelectric point of the protein was 30.535 9 kD and 5.53 respectively.Amino acid sequence homology analysis showed that the deduced amino acid sequence of PbARRO-1 gene had 92% homologies with Malus domestica and 80% homologies with Prunus mume.Quantitative real-time RT-PCR analysis indicated that PbARRO-1 gene expressed in roots，stems，leaves of Pyrus ussuriensis，in leaves its expression level was higher at early stage and lower in later stage;in stems its expression level increased at first and then decreased，and reached peak value at 15 days.The conclusion inferred that PbARRO-1 gene was closely related to root induction.

References:

 

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Last Update: 2017-06-27