|Table of Contents|

Free Amino Acids Content Change of Pleurotus eryngii During Cultivation(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2017年09
Page:
125-129
Research Field:
Publishing date:

Info

Title:
Free Amino Acids Content Change of Pleurotus eryngii During Cultivation
Author(s):
LI Qiaozhen; SHANG Xiaodong; SONG Chunyan; LI Zhengpeng; LI Yan; BAO Dapeng
(Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences/Key Laboratory of Edible Fungi Resources and Utilization(South)，Ministry of Agriculture/National Engineering Research Center of Edible Fungi/National R&D Center for Edible Fungi Processing/Key Laboratory of Agricultural Genetics and Breeding of Shanghai，Shanghai 201403)
Keywords:
Pleurotus eryngii; substrate and mycelium; fruiting body; free amino acids
PACS:
-
DOI:
10.11937/bfyy.201709027
Abstract:
In order to grasp the change regularity of amino acids content in the substrate mycelium and in the fruit body of Pleurotus eryngii during the growth，and lay the foundation for exploring further organic nitrogen carrier and nitrogen transfer method after hypha absorb nitrogen，the amino acids contents were determined in the substrate mycelium and in the fruit body of Pleurotus eryngii at different development stages with automatic analyzer amino acids.The results showed that the content of total free amino acids in the substrate mycelium increased significantly at the stage of spawn running，and decreased gradually at the fruiting stage.The most abundant amino acids in the substrate mycelium was arginine，the content of arginine reached the highest value 7 082.30 μg?g-1 （65.86%） at primordium phase，and gradually declined from the primordium phase to the fruiting body matured stage，which finally was 1 741.31 μg?g-1（40.67%）.This suggested the arginine played an important role in nitrogen transport during the growth of P.eryngii.

References:

 

[1]郭美英.珍稀食用菌杏鲍菇生物学特性的研究[J].福建农业学报,1998,13(3):44-49.

[2]王家季,刘化民.培养料碳氮比对金针菇生长发育及产量的影响[J].中国食用菌,2004(4):30-31.

[3]贾素巧.碳氮营养对猴头菌生长发育及胞外酶活性的影响[D].保定:河北农业大学,2006.

[4]李伟平.碳氮营养对秀珍菇生长发育及胞外酶活性的影响[D].保定:河北农业大学,2007.

[5]JIN H R，PFEFFER P E，DOUDS D D，et al.The uptake，metabolism，transport and transfer of nitrogen in an arbuscular mycorrhizal symbiosis[J].New Phytol,2005,168:301-310.

[6]CRUZ C,EGSGAARD H,TRUJILLO C,et al.Enzymatic evidence for the key role of arginine in nitrogen translocation by arbuscular mycorrhizal fungi[J].Plant Physiology,2007,144:782-792.

[7]JIN H R.Arginine bi-directional translocation and breakdown into ornithine along the arbuscular mycorrhizal mycelium[J].Sci China Ser C-Life Sci,2009,52:381-389.

[8]BORSUK P,DZIKOWSKA A,EMPEL J,et al.Structure of the arginase coding gene and its transcript in Aspergillus nidulans[J].Acta Biochim Pol,1999,46:391-403.

[9]DZIKOWSKA A,KACPRZAK M,TOMECKI R,et al.Specific induction and carbon/nitrogen repression of arginine catabolism gene of Aspergillus nidulans functional in vivo analysis of the otaA promoter[J].Fung Genet Biol,2003,38:175-186.

[10]WAGEMAKER M J M,EASTWOOD D C,van der DRIFT C,et al.Argininosuccinate synthetase and argininosuccinate lyase:Two ornithine cycle enzymes from Agaricus bisporus[J].Mycol Res,2007,111:493-502.

[11]张引芳,刘遐,陈建华,等.杏鲍菇工厂化生产工艺研究[J].食用菌学报,2003,10(2):36-39.

[12]于海龙.可控环境下杏鲍菇子实体发育模型研究[D].南京:南京农业大学,2009:70-81.

Memo

Memo:
-
Last Update: 2017-05-17