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Extraction Technology of Fermentation Substance by Fresh JUNCAO and  Ganoderma lucidum Polysaccharides and Antioxidant Activity in vitro(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2017年09
Page:
120-125
Research Field:
Publishing date:

Info

Title:
Extraction Technology of Fermentation Substance by Fresh JUNCAO and  Ganoderma lucidum Polysaccharides and Antioxidant Activity in vitro
Author(s):
ZHENG Dan1; 2; CHEN Jinhua1; 2; LIN Zhanxi1; 2
(1.National Engineering Research Center of JUNCAO Technology，Fujian Agriculture and Forestry University，Fuzhou，Fujian 350002；2.JUNCAO Research Institute，Fujian Agriculture and Forestry University，Fuzhou,Fujian 350002)
Keywords:
JUNCAO; Ganoderma lucidum mycelium; polysaccharide; extraction process; scavenging rate
PACS:
-
DOI:
10.11937/bfyy.201709026
Abstract:
ermentation substance of fresh JUNCAO and Ganoderma lucidum were used as test material.By single factor test and orthogonal test，the effect of different solid-liquid ratio，extraction time，extraction temperature and extraction times on the yield of polysaccharide extraction were studied，in order to obtain extraction condition optimization.Then by the Fenton system and the pyrogallol autoxidation system，the scavenging rate of fermentation substance of fresh JUNCAO and Ganoderma lucidum to polysaccharide hydroxyl radical and superoxide radical were studied.The results showed that the primary and secondary factors affecting the extraction yield of polysaccharides were extraction temperature>solid liquid ratio>extraction times>extraction time.The optimum extraction conditions were the solid liquid ratio of 1∶30 g?mL-1，temperature 100 ℃，extraction time 3 hours，4 times of extraction，and under the best combination the extraction of polysaccharide,the yield was 4.88%.Fermentation substance polysaccharide had obvious scavenging effect on hydroxyl radical and superoxide anion radical，and with the increase of the polysaccharide density，the clearance ability enhanced.It showed that the density of the fermentation substance polysaccharide had an evident does-effect relationship with the clearance ability of hydroxyl radical and superoxide anion radical.According to the relevant equations，fermentation substance polysaccharide EC50 was 0.461 mg?mL-1，and fermentation substance polysaccharide EC50 was 0.864 mg?mL-1.

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Last Update: 2017-05-17