|Table of Contents|

Cloning and Expression Analysis of Gene Related With  Cytoplasmic Male Sterility in Hot Pepper(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2017年08
Page:
90-95
Research Field:
Publishing date:

Info

Title:
Cloning and Expression Analysis of Gene Related With  Cytoplasmic Male Sterility in Hot Pepper
Author(s):
LIU Kewei1WANG Shubin2DIAO Weiping2GE Wei2
(1.Institute of Botany,Jiangsu Province and Chinese Academy of Sciences,Nanjing,Jiangsu 210014;2.Institute of Vegetable Crops,Jiangsu Academy of Agricultural Sciences,Nanjing,Jiangsu 210014)
Keywords:
peppercytoplasmic male sterility(CMS)orf456QRT-PCRexpression analysis
PACS:
-
DOI:
10.11937/bfyy.201708021
Abstract:
The cytoplasmic male sterile line 21A and its maintainer line 21B of hot pepper were used as test materials,the specific fragment were amplified by using specific primers designed according to the pepper cytoplasmic male sterility associated with gene orf456 between the genomic DNAs of 21A and 21B.And the specific fragment were found only in cytoplasmic male sterile line.Cloning and sequencing analysis indicated that the length of the sequence were 292 bp,named CMS292.The effect of expression level of CMS292 in roots,stems,leaves and flower buds at bud and flowering stages were studied by QRT-PCR method,in order to explore the expression mechanism of male sterility related genes.The results indicated that CMS292 expressed with all tissues tested in cytoplasmic male sterile line.The expression in roots and flower buds were higher than in stems and leaves at bud stage,and the expression in stems and flower buds were lower than in roots and leaves at flowering stage.From bud stage to flowering stage,since the expression were downtrend in roots,stems and flower buds,the leaves expression were rised.It was speculated that CMS292 regulated the expression of protein in anther,causing microspore abortion,which led to cytoplasmic male sterility.

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Last Update: 2017-05-05