|Table of Contents|

Effects of Different Hormones on Regeneration System of ‘Colorful pearl’ Tomato(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2017年08
Page:
85-89
Research Field:
Publishing date:

Info

Title:
Effects of Different Hormones on Regeneration System of ‘Colorful pearl’ Tomato
Author(s):
SUN XiangkeLI YuanyuanLIU XueLI RongyeSU Feng
(School of Chemical Engineering,Qingdao University of Technology,Qingdao,Shandong 266042)
Keywords:
Solanum lycopersicum ‘Colorful pearl’cotyledonhypocotyledonary axisregeneration system
PACS:
-
DOI:
10.11937/bfyy.201708020
Abstract:
‘Colorful pearl’ tomato was used as the test material,cotyledon and hypocotyledonary axis of 6 day-old sterile seedlings were used as explants to study the effect of different hormone concentration and hormone ratio on induction of tomato callus,differentiation of adventitious buds and rooting induction in order to establish regeneration system for tomato.The results showed that the optimum culture medium for induction of tomato callus was MS+0.4 mg?L-1IAA+2.0 mg?L-1 6-BA,in which the induction rate of cotyledon and hypocotyledonary axis was 100% and 95%,respectively.The optimum culture medium for differentiation of adventitious buds was MS+0.2 mg?L-1 IAA+(1.0-3.0)mg?L-1 6-BA;the optimum culture medium for rooting induction was 1/2MS+0.10 mg?L-1 NAA.It concluded that cotyledon was the optimal explant.Its adventitious bud differentiation and growth rates were better than those of hypocotyledonary axis.

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Last Update: 2017-05-05