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Purification Technology of Total Flavonods in Platycodon grandiflorum Stems With Macroporous Resin Methods and Its Antioxidant Ability(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2017年02
Page:
136-141
Research Field:
Publishing date:

Info

Title:
Purification Technology of Total Flavonods in Platycodon grandiflorum Stems With Macroporous Resin Methods and Its Antioxidant Ability
Author(s):
WANG Xiaolin1; JIN Longzhe2; ZHONG Fangli1; XUE Jianfei1; JIA Jiajia1
(1.School of Chemistry and Pharmaceutical Engineering，Jilin Institute of Chemical Technology,Jilin,Jilin 132022；2.Insitute of Processing of Agricultural Products,Yanbian Academy of Agricultural Sciences,Yanji,Jilin 133001）
Keywords:
Platycodon grandiflorum stems; total flavonods; macroporous resin; purification; antioxidant
PACS:
-
DOI:
10.11937/bfyy.201702033
Abstract:
Platycodon grandiflorum stems were used as test materials.The purification technology of total flavonods (TFs) in stems with LSA-21 type macroporous resin methods and its antioxidant ability in vitro were discussed by single factor and orthogonal test.The results indicated that in the condition that the crude drug concentration of sample solution，ratio between sample solution volume and resin mass，batch adsorption time，concentration of eluent，ratio between desorption solution volume and resin mass and vibrating desorption time were 0.06 g?mL-1，20.0 mL?g-1，4.5 hours，70% ethanol solution，27.5 mL?g-1 and 3.5 hours,respectively，the content of TFs in Platycodon grandiflorum stems extracts increased from 6.37% to 14.02%.The TFs in Platycodon grandiflorum stems had strong scavenging activity to DPPH? and ?OH，and the scavenging activity might gradually be enhanced with the increase of mass concentration of TFs in extraction solution.

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Last Update: 2017-03-22