|Table of Contents|

Optimization of Fermentation Conditions and Disease Control Effectiveness  Research of Antagonistic Bacteria TB-12(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2017年15
Page:
36-41
Research Field:
Publishing date:

Info

Title:
Optimization of Fermentation Conditions and Disease Control Effectiveness  Research of Antagonistic Bacteria TB-12
Author(s):
WANG Wei; LI Jia; JIANG Junpo; WANG Shiying
(College of Life Science，Agricultural University of Hebei，Baoding，Hebei 071001)
Keywords:
Botrytis cinerea Pers.; fermentation; antagonistic activity; orthogonal test; control effect
PACS:
-
DOI:
10.11937/bfyy.20165082
Abstract:
Botrytis cinerea was used as indicator bacteria.The fermentation medium and process parameters of strain TB-12 was optimized by single factor experiment and orthogonal test to improve antibacterial effect of strain TB-12.Pot experiments were also carried out to investigate the control efficiency of Botrytis cinerea with fundamental and optimized fermented liquors comparatively.The results showed that optimal medium lactose 2%，casein peptone 4% and MgSO4?7H2O 0.1%，KCl 0.05%.The optimal fermentation conditions were：inoculation amount 10%，volume ratio of seed liquid fermentation medium 50 mL，fermentation time 60 hours，initial pH 8，fermentation temperature 30 ℃，rotation speed 180 r?min-1.Compared with the fermentation of culture medium，the inhibition effect of TB-12 strain on Botrytis cinerea Pers. increased to 69.4%.Pot experiments showed that after optimization the preventive effect of TB-12 strain on the prevention of tomato gray mold was 69.2%，and the treatment effect was 58.2%，which increased by 32.6% and 19.5% respectively.TB-12 strain had good control effect on tomato gray mold.

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Last Update: 2017-08-03