|Table of Contents|

Optimization of SSR-PCR Reaction System forMalus sieversii (Lebed.) Roem.(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2017年15
Page:
29-35
Research Field:
Publishing date:

Info

Title:
Optimization of SSR-PCR Reaction System forMalus sieversii (Lebed.) Roem.
Author(s):
ZHANG Yunxiu1YANG Meiling2YU Weiwei1ZOU Jiahui1LONG Hong1YAN Guorong1
(1.Garden and Horticulture Institute,Tianjin Agricultural University,Tianjin 300384;2.College of Life Sciences,Nankai University,Tianjin 300071)
Keywords:
Malus sieversiiextract genomic DNASSR-PCRoptimization
PACS:
-
DOI:
10.11937/bfyy.20165044
Abstract:
CTAB method was adopted to extract genomic DNA from the dried leaves of Malus sieversii (Lebed.) Roem.The effect of different concentrations of components on SSR-PCR was studied by using control variate method referring to basic PCR system in order to lay the foundation of genetic breeding for Malus sieversii (Lebed.) Roem.The results showed that the optimal SSR-PCR system was 10×PCR buffer 2.5 μL,dNTP 0.2 mmol?L-1,0.2 mmol?L-1 primers,0.5 U Taq DNA polymerase,100 ng DNA template;154 kinds of Malus sieversii germplasm were used to test the stability and universality of system,amplification effect was good.

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Last Update: 2017-08-03