|Table of Contents|

Tissue Culture and Rapid Propagation of Acer rubrum(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2016年20
Page:
97-102
Research Field:
Publishing date:

Info

Title:
Tissue Culture and Rapid Propagation of Acer rubrum
Author(s):
WU Yaqiong1; 2; LIU Jing1; 2; WANG Guibin1; 2; CAO Fuliang1; 2
(1.Co-innovation Center for Sustainable Forestry in Southern China，Nanjing Forestry University，Nanjing，Jiangsu 210037；2.College of Forestry，Nanjing Forestry University，Nanjing，Jiangsu 210037)
Keywords:
Acer rubrum; tissue culture; rooting
PACS:
-
DOI:
10.11937/bfyy.201620025
Abstract:
Taking Acer rubrum as material, using transformation technology，the whole process from the sterile system establishment，start up cultivation，breeding，seedling cultivation，root culture and hardening-seedling transplanting were discussed by different homone proportion.The results showed that the best disinfection way of the stem with bud explants was disinfection by 70% alcohol immersion 30 seconds+6 minutes disinfection by 1% mercuric chloride.The highest survival rate and sprout rate were 75.22% and 95.13%.Adopting orthogonal test，medium and hormone ratio test to start training.The former result showed that the influence of NAA was greater than 6-BA，MS+NAA 0.05 mg?L-1+6-BA 0.5 mg?L-1 had the highest start rate of 62.80%.The latter result showed NN69+2,4-D 0.1 mg?L-1+TDZ 0.3 mg?L-1 had the highest start rate of 80.21%.The best medium for multiplication culture was NN69+NAA 0.1 mg?L-1+TDZ 0.3 mg?L-1，the multiplication factor was 4.2.The MS+2,4-D 0.1 mg?L-1+6-BA 0.5 mg?L-1 could effectively promote the growth of test-tube seedlings.In root culture stage，the optimal culture medium was MS+NAA 0.1 mg?L-1+2,4-D 0.5 mg?L-1.The rooting effect was best with root rate 99.8%，root number 7.9，root length 6.5 cm.Amounts of elements benefits test-tube plant rooting.After the culture-bottle seedlings were transplanted into the matrix composed of vermiculite and perlite (1∶1,V∶V)，the transplanting survival rate was up to 75.2%.

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Last Update: 2016-11-22