|Table of Contents|

Key Technology for Tissue Culture of Bozhou White Peony Root Seedlings(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2016年20
Page:
102-105
Research Field:
Publishing date:

Info

Title:
Key Technology for Tissue Culture of Bozhou White Peony Root Seedlings
Author(s):
LIANG XiaominLUO Ganfeng
(Jiangxi Agricultural Engineering College,Zhangshu,Jiangxi 331200)
Keywords:
Paeonia lactiflora Pall.bulbilsexplants collection timesurface sterilization methodplant tissue culture
PACS:
-
DOI:
10.11937/bfyy.201620026
Abstract:
The bulbils of Bozhou white peony root seedlings were used as explants for the trial of Bozhou peony tissue culture.Based on 3 stages of sampling,the best time for explants collection was optimized.Based on the bulbils collected in the last tenday of February and the first tenday of March,5 surface sterilizing conditions and 3 plant hormones IBA,NAA and BA at different levels of combinations with the experimental design of 4 factors and 2 levels orthogonal test were investigated.The BA and KT combinations with the experimental design of 2 factors and 2 levels orthogonal test were also optimized to establish the fast propagation technology for Bozhou white peony root seedlings.The results showed that the explants collected in the last tenday of February and the first tenday of March had the highest rate of induction.The best surface sterilizing condition was submersing the bulbils for 0.13% HgCl2 for 10 minutes and then repeated again.The optimized initiation culture medium for bulbils was 1/2 MS+1.0 mg?L-1 BA+0.5 mg?L-1 KT+3.0% sucrose+0.7% agar.

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Last Update: 2016-11-22