Sensitivity of Osmanthus fragrans Seeds to 60Co-γ Radiation and Its Medial Lethal Doses in Radiation Breeding(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Sensitivity of Osmanthus fragrans Seeds to 60Co-γ Radiation and Its Medial Lethal Doses in Radiation Breeding

Author(s):: XIONG Yunhai; WAN Lusheng; (School of Forestry and Life Sciences,Chongqing University of Arts and Sciences,Chongqing 402160)

Keywords:: Osmanthus fragrans(Thumb.)L.; radiation breeding; medial lethal dose

Abstract:: In order to study the radiative mutation breeding of Osmanthus fragrans，explore the radiation dosages of Osmanthus fragrans and select new variety of Osmanthus fragrans having high ornamental value，the Osmanthus fragrans seeds from two different varieties(var.thunbergii Makino and var.latifolius Makino) were treated with different dosages of 60Co-γ ray (0,50,100，150，200，250 and 300 Gy)，and the seed germination rate and radicle growth were observed.Based on the germination rate 50%，LD50 doses of 60Co-γ radiation for the seeds were calculated using linear regression equation.The results showed that 60Co-γ radiation caused the seeds radicle length shortened，lower germination rate.LD50 doses of 60Co-γ radiation for the seeds of var.thunbergii Makino and var. latifolius Makino were 123.807 Gy and 118.381 Gy respectively.Their maximum radiation dose was 250 Gy.The seeds germination rate of Osmanthus fragrans had a high negative correlation with radiation dose，the correlation coefficient was r=-0.910，r=-0.934,respectively.The radiation sensitivity of var.thunbergii Makino seeds was higher than var.latifolius Makino seeds.

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Last Update: 2016-11-22