|Table of Contents|

Transformation Techniques for Petunia hybrida With ACC Deaminase Gene Driven by Different Promoters(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2016年20
Page:
85-91
Research Field:
Publishing date:

Info

Title:
Transformation Techniques for Petunia hybrida With ACC Deaminase Gene Driven by Different Promoters
Author(s):
LI Jianyong1; 2
(1.College of Jiasixie Agriculture,Weifang University of Science and Technology，Shouguang，Shandong 262700;2.Laboratory of Plant Improvement and Utilization，Yunnan University，Kunming，Yunnan 650091)
Keywords:
Petunia hybrida;  promoter; ACC deaminase gene; genetic transformation
PACS:
-
DOI:
10.11937/bfyy.201620023
Abstract:
Taking Petunia hybrida as material，ACC deaminase gene(ACDS) (driven respectively by promoter CaMV35S，SAG12 and CHSA) was transformed into Petunia hybrida leaves，mediated by Agrobacterium tumefaciens.The effects of the pre-culture time，concentration of Agrobacterium tumefaciens，infection time，co-culture time and concentration of AS on transformation were studied.The transformation efficiency of ACDS gene driven by different promoters was compared based on the percentage of transgenic plants and the growth rate of transgenic plants.The results showed that the best transformation efficiency was abtained under the conditions of Petunia hybrida leaves pre-culture for 5 days，infection for 8 minutes in Agrobacterium tumefaciens liquid of 0.6 OD600 value containing 200 μmol?L-1 acetosyringone，and then co-culture for 4 days.ACDS gene driven by CHSA promoter had the highest transformation efficiency(3%），while ACDS gene driven by SAG12 promoter had the lowest transformation efficiency(1.25%).PCR analysis verified that the foreign gene was integrated into the Petunia hybrida genome.The obtaining of Petunia hybrida transgenic plants set up a technical basis for breeding of anti-aging Petunia hybrida varieties via genetic engineering.

References:

 

[1]JOHN C T，ANN M A，GLENN A.The indole alkaloid tryptamine produced in transgenic Petunia hybrida[J].Plant Physiology and Biochemistry，1999，37(9)：665-670.

[2]MEYER P，HEIDMANN I，FORKMANN G.A new petunia flower color generated by transformation of a mutant with a maize gene[J].Nature，1987，333：677-678.

[3]褚云霞，潘俊松，吴爱忠,等.矮牵牛花色基因工程研究进展[J].西北植物学报，2013，33(2):422-428.

[4]余勇，葛文东，邹世慧，等.异源表达拟南芥赤霉素2-氧化酶基因对矮牵牛形态发育的影响[J].园艺学报,2014,41(10):2065-2074.

[5]HONMA M,SHIMOMURA T.Metabolism of 1-aminocyclopropane-1-carboxylic acid[J].Agricultural and Biological Chemistry，1978，42：1825-1931.

[6]王海滨，王平，陈永华.ACC脱氨酶的作用机理和转基因的应用[J].生物技术通报，2009，12(4):40-43.

[7]KLEE H J，HAYFORD M B，KRETZMER K A.Control of ethylene synthesis by expression of a bacterial enzyme in transgenic tomato plants[J]. Plant Cell，1991，3：1187-1193.

[8]黄海泉，黄美娟，丁小维，等.阴沟肠杆菌UW4菌株ACC脱氨酶基因的克隆、序列分析及原核表达[J].农业生物技术学报，2006，14(6)：952-956.

[9]秦智慧，刘艳昆，孙彦.不同启动子驱动下ACDS基因转化烟草及耐盐性研究[J].生物技术通报，2010，16(10):120-125.

[10]马学军，舒跃龙，颜子颖,等.精编分子生物学实验指南[M].4版.北京：科学出版社，2005：53-58.

[11]曹冬梅，康黎芳，李永平，等.安祖花Arizona 品种遗传转化体系的优化及矮牵牛查尔酮合成酶基因(PhCHS)的导入[J].农业生物技术学报，2015，23(3)：329-336.

[12]刘南南，王宝琴，管宇.根癌农杆菌介导反义F3′H基因对矮牵牛的遗传转化[J].广东农业科学，2014，39(6)：147-149.

[13]KUMAR S V，RAJAM M V.Polyamines enhance Agrobacterium tumefaciens vir gene induction and T-DNA transfer[J]. Plant Science，2005,168(2)：475-480.

[14]郑楠，王霞霞，曹冬梅.农杆菌介导萱草植株CHS基因的转化[J].中国园艺文摘，2015，12(2)：5-11.

[15]李莉,祁银燕,解燕,等.矮牵牛编码F3′5′H的蓝色基因表达载体构建及转化[J].西北植物学报，2011，31(6):1090-1096.

[16]MIKHAJLO K,ZUBKO,ELENA I，et al.Stable transformation of Petunia plastids[J].Transgenic Research,2004，13：523-530.

Memo

Memo:
-
Last Update: 2016-11-22