|Table of Contents|

Cloning and Bioinformatics Analysis of the GBSS Gene From Chinese Chestnut(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2016年16
Page:
99-103
Research Field:
Publishing date:

Info

Title:
Cloning and Bioinformatics Analysis of the GBSS Gene From Chinese Chestnut
Author(s):
WANG MengLIANG Xue
(College of Forestry,Southwest Forestry University,Kunming,Yunnan 650224)
Keywords:
Castanea mollssinagranule-bound starch synthaseclonesequence analysis
PACS:
-
DOI:
10.11937/bfyy.201616026
Abstract:
Taking chestnut as test material,GBSS gene was cloned from the first strand of Castanea mollssina seed’s cDNA through RT-PCR methods,and the bioinformatics characters were analysed.The results showed that a full length cDNA of Cgbss(GenBank accession number:KU162945) was obtained.The length of Cgbss gene was 2 085 bp,which contained an open reading frame encoding 611 amino acids.The bioinformatics analysis indicated that the pI and MW of animo acids encoded by Cgbss were predicted to be 8.36 and 67.580 9 kDa,respectively,and the protein had one GT1_glycogen_synthase domain.And the fragment had 85% homology identical to the already reported sequence of GBSS I.

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Last Update: 2016-09-18