|Table of Contents|

Transferability and Application of Brassica SSR Markers in Flowering Chinese Cabbage(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2016年15
Page:
86-91
Research Field:
Publishing date:

Info

Title:
Transferability and Application of Brassica SSR Markers in Flowering Chinese Cabbage
Author(s):
CHEN Jingfang1LI Ronghua1WANG Zhiliang1XIA Yanshi1GUO Peiguo1Kadambot SIDDIQUE2
(1.International Crop Research Center for Stress Resistance/College of Life Sciences,Guangzhou University,Guangzhou,Guangdong 510006;2.Institute of Agriculture,University of Western Australia,WA 6009,Australia)
Keywords:
BrassicaSSRtransferabilityflowering Chinese cabbagegenetic linkage group
PACS:
-
DOI:
10.11937/bfyy.201615022
Abstract:
In order to develop SSR markers suitable for flowering Chinese cabbage,four flowering Chinese cabbage genotypes different in genetic background were selected to detect the amplification effects of 133 Brassica SSR primers.The results showed that 50 of 133 Brassica SSR primer combinations were confirmed to have the property of transferability in flowering Chinese cabbage.The amplicons of these SSR mainly ranged from 100 to 350 bp.91 polymorphic loci among 4 genotypes were detected with 1 to 4 polymorphic locus/loci per SSR primer pair.The population containing 130 recombinant inbred lines from the cross of ‘Shijiu-19 Caixin’ and ‘3T6’ was genotyped by using the SSR primer combinations which producing polymorphic loci between parents,and a genetic linkage map containing 10 linkage groups was established with a length of 641.39 cM.These results could be beneficial for the genetic diversity analysis,QTL mapping and marker-assisted selection in breeding program of flowering Chinese cabbage.

References:

 

[1]许兰桂,夏岩石,李荣华,.菜薹分子标记利用的研究进展[J].园艺学报,2012,39(9):1739-1748.

[2]郭培国,许兰桂,何嘉仪,.菜心种质材料的分子身份证构建研究[J].广东农业科学,2014,41(23):116-122.

[3]郭培国,许兰桂,夏岩石,.菜薹种质遗传多样性的荧光MFLP标记分析[J].园艺学报,2015,42(2):350-360.

[4]POWELL W,MACHRAY G C,PROVAN J.Polymorphism revealed by simple sequence repeats[J].Trends in Plant Science,1996,1(7):215-222.

[5]MCGREGOR C E,LAMBERT C A,GREYLING M M,et al.A comparative assessment of DNA fingerprinting techniques (RAPDISSRAFLP and SSR) in tetraploid potato(Solanum tuberosum L.)germplasm[J].Euphytica,2000,113(2):135-144.

[6]罗丹,吴委林,周波,.芜菁SSR引物的开发及多态性分析[J].生物技术通讯,2012,23(3):402-406.

[7]王炜勇,俞少华,张飞,.近缘属SSR标记引物在光萼荷属植物中的通用性[J].分子植物育种,2013,11(4):583-587.

[8]CARVALHO S I,RAGASSI C F,OLIVEIRA I B,et al.Transferability of microsatellite markers of Capsicum annuum L.to C.frutescens L.and C.chinense Jacq[J].Genetics and Molecular Research,2015,14(3):7937-7946.

[9]龚亚明,徐盛春,毛伟华,.豌豆EST-SSR标记在蚕豆中的通用性与应用[J].浙江大学学报(农业与生命科学版),2011,37(5):479-484.

[10]荆赞革,唐征,罗天宽,.甘蓝SSR标记在近缘种青花菜的通用性及其应用[J].基因组学与应用生物学,2010,29(4):685-690.

[11]刘海霞,颉建明,康俊根.芸薹属SSR引物在甘蓝分子育种中通用性的研究[J].北方园艺,2010(3):115-118.

[12]李荣华,夏岩石,刘顺枝,.改进的CTAB提取植物DNA方法[J].实验室研究与探索,2009,28(9):14-16.

[13]SUWABE K,IKETANI H,NUNOME T,et al.Isolation and characterization of microsatellites in Brassica rapa L.[J].Theoretical Applied Genetics,2002,104(6-7):1092-1098.

[14]SUWABE K,TSUKAZAKI H,IKETANI H,et al.Identification of two loci for resistance to clubroot(Plasmodiophora brassicae Woronin)in Brassica rapa L.[J].Theoretical and Applied Genetics,2003,107(6):997-1002.

[15]LOWE A J,JONES A E,RAYBOULD A F,et al.Transferability and genome specificity of a new set of microsatellite primers among Brassica species of the U triangle[J].Molecular Ecology Notes,2002,2(2):7-11.

[16]LOWE A J,MOULE C,TRICK M,et al.Efficient large-scale development of microsatellites for marker and mapping applications in Brassica crop species[J].Theoretical and Applied Genetics,2004,108(6):1103-1112.

[17]PIQUEMAL J,CINQUIN E,COUTON F,et al.Construction of an oilseed rape (Brassica napus L.) genetic map with SSR markers[J].Theoretical and Applied Genetics,2005,111(8):1514-1523.

[18]CHOI S R,TEAKLE G R,PRIKSHIT P,et al.The reference genetic linkage map for the multinational Brassica rapa genome sequencing project[J].Theoretical and Applied Genetics,2007,115(6):777-792.

[19]于仁波,于拴仓,戚佳妮,.大白菜SSR锚定标记分子遗传图谱构建[J].园艺学报,2008,35(10):1447-1454.

[20]SUWABE K,IKETANI H,NUNOME T,et al.Characteristics of microsatellites in Brassica rapa genome and their potential utilization for comparative genomics in Cruciferae[J].Breeding Science,2004,54(2):85-90.

[21]SINGH B K,THAKUR A K,TIWARI S K,et al.Transferability of Brassica-derived microsatellites to related genera and their implications for phylogenetic analysis[J].National Academy Science Letters,2012,35(1):37-44.

[22]忻雅,崔海瑞,张明龙,.白菜EST-SSR标记的通用性[J].细胞生物学杂志,2006(28):248-252.

[23]王嘉,荆凌云,荐红举,.甘蓝型油菜株高、第一分枝高和分枝数的QTL检测及候选基因筛选[J].作物学报,2015,41(7):1027-1038.

[24]高会超,曾强,张志刚,.与大白菜TuMV抗病基因TuRBCS01紧密连锁的分子标记开发[J].农业生物技术学报,2016,24(2):196-205.

[25]李媛媛,沈金雄,王同华,.利用SRAPSSRAFLP标记构建甘蓝型油菜遗传连锁图谱[J].中国农业科学,2007,40(6):1118-1126.

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Last Update: 2016-08-29