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Optimizing of Culture Medium of Agaricus bisporus (Lange) Imbach for  Extracellular polysaccharide Production by Response Surface Methodology(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2016年12
Page:
152-157
Research Field:
Publishing date:

Info

Title:
Optimizing of Culture Medium of Agaricus bisporus (Lange) Imbach for  Extracellular polysaccharide Production by Response Surface Methodology
Author(s):
YU Mei1; JIAO Yingchun2; SUN Xu2; YU Gang2; QIU Dongmei2; GAO Shumin3
(1.Qinghai Plateau Pharmaceutial Co.Ltd.,Xining,Qinghai 810007;2.College of Agriculture and Animal Husbandry,Qinghai University,Xining,Qinghai 810016;3.Agro-forestry Academy of Sciences,Xining,Qinghai 810016)
Keywords:
response surface methodology; Agaricus bisporus (Lange) Imbach; extracellular polysaccharide; culture medium; optimizing
PACS:
-
DOI:
10.11937/bfyy.201612038
Abstract:
Taking Agaricus bisporus (Lange) Imbach as material,the content of extracellular polysaccharide as the response value,on the basis of single factor experiment,the effect of different nutritional factors on the content of extracellular polysaccharide were researched by the response surface methodology with 4 factors and 4 levels to determined the optimum culture medium.The results showed that the best culture medium for fermentation extracellular polysaccharide of the Agaricus bisporus (Lange) Imbach was mannitol 19.83 g?L-1,peptone 6.35 g?L-1,vitamin B12 1.61 g?L-1,iron sulfate 0.42 g?L-1,the highest production of extracellular polysaccharide reached 2.002 g?L-1,which was identical to the response surface prediction value.

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Last Update: 2016-07-21