|Table of Contents|

Research on Wild Almond Self-incompatibility Related Gene PetSSK1 Transferring Into Petunia ‘Dream’(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2016年12
Page:
96-100
Research Field:
Publishing date:

Info

Title:
Research on Wild Almond Self-incompatibility Related Gene PetSSK1 Transferring Into Petunia ‘Dream’
Author(s):
XIA Jianghong1; ZENG Bin1; WANG Jianyou2; LI Weiyang1; TIAN Jia1; LI Jiang1
(1.Collage of Forestry and Horticulture/The Characteristics Fruit Tree Research Center,Xinjiang Agricultural University,Urumqi，Xinjiang 830052;2.Extension Station,Xinjiang Academy of Forestry Sciences,Urumqi,Xinjiang 830063)
Keywords:
wild almond(Prunus tenella); PetSSK1; plant expression vector; Agrobacterium tumefaciens; Petunia hybrida
PACS:
-
DOI:
10.11937/bfyy.201612024
Abstract:
Taking the pollen of Xinjiang wild almond(Prunus tenella) as material,the expression plasmid pCAMBIA1303-PetSSK1 with self-incompatibility related gene PetSSK1 was constructed.Then it was transferred into Petunia mediated by Agrobacterium tumefaciens,and the effect of different kinds and concentration of antibiotics on Petunia regeneration was studied.The results showed that the Petunia taking self-incompatibility related gene PetSSK1 of Xinjiang wild almond was gotten successfully.The sensitive concentration of Kan and Cef were 25 mg?L-1 and 300 mg?L-1,respectively.And the Petunia were further confirmed as positive plants by PCR detection.

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Last Update: 2016-07-21