|Table of Contents|

Orthogonal Design-direct Analysis of SSR-PCR Reaction System in Momordica charantia(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2016年10
Page:
99-103
Research Field:
Publishing date:

Info

Title:
Orthogonal Design-direct Analysis of SSR-PCR Reaction System in Momordica charantia
Author(s):
WANG Xindi1; 2; 3; HUANG Rukui2; 3; FENG Chengcheng2; 3; LIANG Jiazuo2; HUANG Xiongjuan2; 3; LIU Xinglian2
(1.College of Agriculture，Guangxi University，Nanning，Guangxi 530001；2.Vegetable Research Institute，Guangxi Academy of Agricultural Sciences，Nanning，Guangxi 530007；3.The Key Laboratory of Crops Genetic Improvement Biological Technology of Guangxi，Nanning，Guangxi 530007)
Keywords:
Momordica charantia; orthogonal experiment; single factor design
PACS:
-
DOI:
10.11937/bfyy.201610025
Abstract:
Taking Momordica charantia ‘MC9’ as test material,an orthogonal experiment design of L16(45) with four levels and five factors was carried out，the factors were the concentration of Mg2+，the concentration of dNTPs，Taq polymerase，DNA template and the concentration of primers.The concentration of Mg2+，the concentration of dNTPs and DNA polymerase using single factor experiment design were also determined.The results showed that the optimum system containing 1 μL 10×PCR buffer,Mg2+ 1.75 mmol?L-1，dNTPs 0.25 mmol?L-1，Taq DNA polymerase 1 U，DNA template 100 ng，primer 0.20 mmol?L-1.

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Memo

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Last Update: 2016-07-11