Study on High Quality Plantlets Propagation Technique of Dendrobium officinale(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Study on High Quality Plantlets Propagation Technique of Dendrobium officinale

Author(s):: ZHOU Li1; WANG Yuan1; YE Yuanting1; XU Zhenghai2; （1.Key Laboratory of National Medicinal and Biological Resources，Xingyi Normal University for Nationalities,Xingyi，Guizhou 562400；2.Southwest Guizhou Lyuyuan Animal and Plant Technologies Co.Ltd.，Xingyi，Guizhou 562400)

Keywords:: Dendrobium officinale; seed; asymbiotic germination; high quality plantlets

Abstract:: Taking wild Dendrobium officinale as test material,using root restriction culture method，a high quality plantlets breeding technology of Dendrobium officinale was introduced by researching its characteristics of the asymbiotic seed germination.The results showed that the seeds of 120 days after pollination (DAP) reached maturity，the seeds could germinate；the seeds of 140 DAP could be morphologically mature，with a seed germination rate up to 98.51%；low temperature and darkness restriction could inhibit of protocorms growth；using medium of restrictions on root growth in a particular container could get high quality seedlings，restriction on root growth medium was MS+0.10 mg?L-1 6-BA+0.3 mg?L-1 NAA+80 g?L-1 mashed banana；and the survival rate could be up to 97.62% when the plantlets were transplanted to pine bark.Therefore，this research provided a new method for the industrial production of D.officinale

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Last Update: 2016-07-11