|Table of Contents|

Pathogenicity Mechanism of Yang Mei Shuang Disease on Tremella fuciformis and Inhibition Spectrum of the Pathogenic Bacterium(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年24
Page:
114-117
Research Field:
Publishing date:

Info

Title:
Pathogenicity Mechanism of Yang Mei Shuang Disease on Tremella fuciformis and Inhibition Spectrum of the Pathogenic Bacterium
Author(s):
LI Bingbing1; 2; WEN Zhiqiang1
(1.College of Life Science,Fujian Agriculture and Forestry University，Fuzhou，Fujian 350002;2.Huai’an Center for Disease Control and Prevention，Huai’an,Jiangsu 223001)
Keywords:
Yang mei shuang disease on Tremella fuciformis; pathogenic bacterium; inhibition spectrum
PACS:
-
DOI:
10.11937/bfyy.201524032
Abstract:
Taking Tremella fuciformis artificial bed-log which was infected with Yang mei shuang disease as materials，plate cultivation and molecular biology methods were used to study the biological characteristics and antibacterial spectrum of pathogenic bacterium.The results showed that the colonies characteristics and cultural characteristics of the three pathogen strains Js-1T，Js-2 and Js-3 were the same as PDA culture.And the 16S rDNA of three strains had the same bands after digestion.，so these strains were repeated strains.Strain Js-1T was confirmed to be the pathogen of this disease through Koch’s rules.Strain Js-1T had strong inhibition on xiang hui and tremella gemma，which could be the cause of the disease.16S rDNA squence analysis showed that strain Js-1T had the highest similarity with Streptomyces hebeiensis strain NBRC 101006T，reaching 97.62%.The results of cultural characteristics and molecular biological analysis showed that strain Js-1T belonged to Streptomyces.What’s more，the antibacterial activity of strain Js-1T was determined.The results showed that strain Js-1T had strong inhibition against these tested strains.The diameters of Escherichia coli and Bacillus subtilis were 2.76 cm and 1.26 cm,respectively.The diameters of Sclerotinia sclerotiorum，Mycogone perniciosa，Pyricularia grisea，Trichoderma viride，Rhizopus stolonir were 4.29，4.07，3.85，3.37 and 2.90 cm,respectively.In addition，strain Js-1T had antibacterial activity to Fusarium spp and Banana vasicular wilt.

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Last Update: 2016-02-17