Optimization of ISSR on Prunus mira koehne Kov et.Kpst(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Author(s):: MENG Fanjuan1; XING Chun1; XU Fuling1; WANG Zhongkui2; LI Rongqin2; (1.College of Life Science，Northeast Forestry University，Harbin，Heilongjiang 150040;2.College of Agriculture and Animal Husbandry Tibet University，Linzhi，Tibet 860000)

Abstract:: To ensure the stability of ISSR for Prunus mira koehne Kov et.Kpst,total genomic DNA was extracted by improved CTAB method，the factors including template DNA concentration,MgCl2 concentration,dNTPs concentration,Taq polymorphism dose and primer concentration were studied to establish optimized ISSR reaction system.The result showed that template concentration 40 ng/μL,primer concentration 0.4 μmol/L,Mg2+ concentration 2.5 mmol/L,enzyme concentration 0.5 U,dNTPs concentration 0.5 mmol/L were the optimized reaction system.The study would provide the foundation for the genetic diversity on germplasm resources of Prunus mira koehne Kov et.Kpst.

[1]陈巍，王力荣，张绍铃，等.利用SSR研究不同国家桃育成品种的遗传多样性[J].果树学报,2007,24(5):580-584.

[2]罗冉，吴委林，张旸，等.SSR分子标记在作物遗传育种中的应用[J].基因组学与应用生物学,2010,29(1):137-143.

[3]罗海燕，陈业渊.ISSR分子标记及其应用[J].安徽农学通报,2008(19):45-46,27.

[4]朱岩芳，祝水金，李永平，等.ISSR分子标记技术在植物种质资源研究中的应用[J].种子,2010(2):55-59.

[5]韩宏.分子标记在果树种质资源研究中的应用[J].陕西农业科学,2010(4):74-76.

[6]余贤美，艾呈祥.杧果野生居群遗传多样性ISSR 分析[J].果树学报,2007(3):329-333.

[7]叶春海，王耀辉，李映志，等.菠萝蜜遗传多样性的ISSR 分析[J].果树学报,2009(5):659-665.

[8]刘威生，冯晨静，杨建民，等.杏ISSR 反应体系的优化和指纹图谱的构建[J].果树学报，2005(6):626-629.

[9]陈少瑜，杨恩，习学良，等.云南主要核桃品种的ISSR分子鉴别[J].经济林研究，2006(4)：41-45.

[10]姜静，杨传平，刘桂丰，等.桦树ISSR-PCR反应体系的优化[J].生态学杂志，2003(3):91-93.

[11]谢运海，夏德安，姜静，等.利用正交设计优化水曲柳ISSR-PCR反应体系[J].分子植物育种，2005(3):445-450.

[12]邹枚伶，夏志强，王文泉.白木香基因组DNA提取与ISSR反应体系的优化[J].中国农学通报,2009(2)：250-254.

[13]刘威生，冯晨静，杨建民，等.杏ISSR 反应体系的优化和指纹图谱的构建[J]．果树学报,2005(6):626-629.

Last Update: 2015-11-19